Mouse Monoclonal to CD253 / TRAIL
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Human CD253 / TRAIL
(TNF-Related Apoptosis Inducing Ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2).
The antibody 2E5 reacts with TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.
Recombinant soluble fragment (aa 95-281) of human TRAIL.
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
*Plasilova M, Zivny J, Jelinek J, Neuwirtova R, Cermak J, Necas E, Andera L, Stopka T: TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia
progenitors. Leukemia. 2002 Jan;16(1):67-73.
*Hyer ML, Croxton R, Krajewska M, Krajewski S, Kress CL, Lu M, Suh N, Sporn MB, Cryns VL, Zapata JM, Reed JC: Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res. 2005 Jun 1;65(11):4799-808.
[Abstract] [Full Text]
For research use only. Not for drug, diagnostic or other use.
Mouse IgG1 Isotype Control
Fig. 1. Induction of apoptosis
Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5
The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL.
A - medium
B - recombinant TRAIL
C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 μg/ml)
D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 μg/ml)
E - recombinant TRAIL + Isotype mouse IgG1 control