Mouse Monoclonal to Daxx / DAP6
Technical Information Request Inquiry for Bulk Prices
- In stock! Ready for immediate despatch
- Manufacture on order
is an apoptosis-modulating death domain-associated protein with functions in transcriptional regulation. Daxx functions bot in cytoplasm, where it interacts with Fas, and in nucleus (residing in PML oncogenic domains), where it is involved in SUMO-dependent regulation of transcription and subnuclear compartmentalization. Daxx senzitizes the cells to apoptosis, but on the other hand, this protein may also serve in preventing apoptosis in the early embryo. Even regarding the transcription, Daxx can serve both as a corepressor and a coactivator. During ischemic stress, Daxx translocates from the nucleus to the cytoplasm, where in regulates sodium hydrogen exchanger isoform 1 (NHE1).
The antibody DAXX-01 reacts with Daxx, a death domain containing protein mainly expressed in fetal and adult human and mouse tissue.
Recombinant C-terminal part (aa 558-740) of human Daxx.
- Western Blotting
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
*Leal-Sanchez J, Couzinet A, Rossin A, Abdel-Sater F, Chakrabandhu K, Luci C, Anjuere F, Stebe E, Hancock D, Hueber AO.: Requirement for Daxx in mature T-cell proliferation and activation. Cell Death Differ. 2007 Apr;14(4):795-806. [Abstract]
For research use only. Not for drug, diagnostic or other use.
Mouse IgG1 Isotype Control
Fig. 1B Immunofluorescence staining (human cervix carcinoma cells)
Fig. 1A Immunofluorescence staining (human cervix carcinoma cells)
Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell line.
Myc Daxx (green) was stained with anti-human Daxx (DAXX-01
), nuclei were stained with DAPI (blue).1A
- nuclear localization of Daxx in HeLa cells transfected with pCDNA3-MycDaxx1B
- HeLa cells were co-transfected with pCDNA3-MycDaxx and pCDNA3-ASK1HA, which led to translocation of Daxx from nucleus to cytoplasm