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Mouse Monoclonal to p21Waf1

WA-1 (IgG1)

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  11-338-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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The tumour suppressor p21Waf1 (Wild-type p53-Activated Fragment 1; also known as Cip1, Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which is expressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors upon various stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibits Cdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA (proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21 counteracts its inhibitory activities.


The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.

Regulatory Status


Human p21 protein

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:


  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    Positive tissue: colon carcinoma
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Waga S, Hannon GJ, Beach D, Stillman B: The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA. Nature. 1994 Jun 16;369(6481):574-8. [Abstract]
  • *Horky M, Kuchtickova S, Vojtesek B, Kolar F: Induction of cell-cycle inhibitor p21 in rat ventricular myocytes during early postnatal transition from hyperplasia to hypertrophy. Physiol Res. 1997;46(3):233-5. [Abstract]
  • *Zhang C, Kavurma MM, Lai A, Khachigian LM: Ets-1 protects vascular smooth muscle cells from undergoing apoptosis by activating p21WAF1/Cip1: ETS-1 regulates basal and and inducible p21WAF1/Cip: ETS-1 regulates basal and inducible p21WAF1/Cip1 transcription via distinct cis-acting elements in the p21WAF/Cip1 promoter. J Biol Chem. 2003 Jul 25;278(30):27903-9. [Abstract] [Full Text]
  • *Keeshan K, Cotter TG, McKenna SL: Bcr-Abl upregulates cytosolic p21WAF-1/CIP-1 by a phosphoinositide-3-kinase (PI3K)-independent pathway. Br J Haematol. 2003 Oct;123(1):34-44. [Abstract]
  • *Choi BH, Kim CG, Bae YS, Lim Y, Lee YH, Shin SY: p21 Waf1/Cip1 expression by curcumin in U-87MG human glioma cells: role of early growth response-1 expression. Cancer Res. 2008 Mar 1;68(5):1369-77. [Abstract]
  • Product Specific References

  • *Kolar Z, Murray PG, Scott K, Harrison A, Vojtesek B, Dusek J: Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer. Mol Pathol. 2000 Feb;53(1):15-8. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    Fig. 1.

    Fig. 1. Immunohistochemistry staining of colon carcinoma (paraffin-embedded sections) with anti-p21 (WA-1).

    p21 fibr
    Fig. 2.

    Fig. 2. Immunofluorescence staining of p21 in human primary fibroblasts using anti-p21 (WA-1; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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