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Mouse Monoclonal to Cdk1 / p34Cdc2

POH-1 (IgG2a)

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Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.


The antibody POH-1 specifically detects Cdk1 (p34Cdc2). Staining of Cdk1 reflects the proliferating potential of respective tissue.

Regulatory Status


Bacterially expressed full-length human Cdk1 protein

Species Reactivity:

  • Human
  • Non-Human Primates
  • Bovine

Negative Species:

  • Mouse
  • Rat
  • Xenopus
  • Drosophila


  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
    Positive tissue: thymus
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S: An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol. 2007 Jul 16;178(2):257-68. [Abstract] [Full Text]
  • *Lindqvist A, van Zon W, Karlsson Rosenthal C, Wolthuis RM: Cyclin B1-Cdk1 activation continues after entrosome separation to control mitotic progression. PLoS Biol. 2007 May;5(5):e123. [Abstract] [Full Text]
  • *Liu ZG, Zhao LN, Liu YW, Li TT, Fan DM, Chen JJ: Activation of Cdc2 contributes to apoptosis in HPV E6 expressing human keratinocytes in response to therapeutic agents. J Mol Biol. 2007 Nov 23;374(2):334-45. [Abstract] [Full Text]
  • Product Specific References

  • *Lukas J, Draetta G, Bartek J: Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur J Biochem. 1992 Jul 1;207(1):169-76. [Abstract]
  • *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2a Isotype Control
  • Example Data

    POH-1 IHC
    Fig. 1.

    Fig. 1. Immunohistochemistry staining of human thymus (paraffin sections) using anti-Cdk1 (clone POH-1).
    Commercially tested by LifeSpan BioSciences.

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