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Mouse Monoclonal to 5-bromodeoxyuridine (BrdU)

MoBu-1 (IgG1)

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Background

Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.

Specificity

The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU).

Regulatory Status

Immunogen

5-bromodeoxyuridine conjugated with hemocyanine.

Species Reactivity:

Negative Species:

Applications:

  • Immunohistochemistry (paraffin sections)
    Application note: excellent
  • Flow Cytometry
    Recommended dilution:1-2 μg/ml
  • Immunocytochemistry
    Recommended dilution: 2 µg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Ashby J, Tinwell H, Soames A, Foster J: Induction of hyperplasia and increased DNA content in the uterus of immature rats exposed to coumestrol. Environ Health Perspect. 1999 Oct;107(10):819-22. [Abstract] [Full Text]
  • *Soames AR, Lavender D, Foster JR, Williams SM, Wheeldon EB: Image analysis of bromodeoxyuridine (BrdU) staining for measurement of S-phase in rat and mouse liver. J Histochem Cytochem. 1994 Jul;42(7):939-44. [Abstract]
  • Product Specific References

  • *Buckiova D, Kubinova L, Soukup A, Jelinek R, Brown NA: Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects. Int J Dev Biol. 1998 Jul;42(5):737-40. [Abstract]
  • *Stanek D, Kiss T, Raska I: Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription. Eur J Cell Biol. 2000 Mar;79(3):202-7. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MoBu-1
    Fig. 1. Flow Cytometry analysis


    Fig. 1. Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute lymphoblastic leukemia cell line using purified anti-5-bromodeoxyuridin (MoBu-1) (detection by Goat anti-mouse IgG1 FITC).
    The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.








    MoBu-1
    Fig. 2. Immunohistochemistry of paraffin-embedded sections (chick embryo)


    Fig. 2. Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) with anti-5-bromodeoxyuridine (MoBu-1).

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