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Mouse Monoclonal to NHERF1 / EBP50

EBP-10 (IgG2b)

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NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.


The antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.

Regulatory Status


Bacterially produced recombinant full-length human NHERF1.

Species Reactivity:

  • Human
  • Other not tested

Negative Species:


  • Immunoprecipitation
  • Western Blotting
    Recommended dilution: 2 μg/ml, 60 min on vertical incubator
    Positive control:
    RAJI human lymphoma cell lysate
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
    Application note: Non-reducing conditions. 10% separating SDS-PAGE gel.
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 5 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Brdickova N, Brdicka T, Andera L, Spicka J, Angelisova P, Milgram SL, Horejsi V.: Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett. 2001 Oct 26;507(2):133-6. [Abstract]
  • *Lederer ED, Khundmiri SJ, Weinman E.J.: Role of NHERF-1 in regulation of the activity of Na-K ATPase and sodium-phosphate co-transport in epithelial cells. J Am Soc Nephrol. 2003 Jul;14(7):1711-9. [Abstract] [Full Text]
  • *Weinman EJ, Cunningham R, Wade JB, Shenolikar S.: The role of NHERF-1 in the regulation of renal proximal tubule sodium-hydrogen exchanger 3 and sodium-dependent phosphate cotransporter 2a. J Physiol. 2005 Aug 15;567(Pt 1):27-32. [Abstract] [Full Text]
  • *Wheeler D, Sneddon WB, Wang B, Friedman PA, Romero G.: NHERF-1 and the cytoskeleton regulate the traffic and membrane dynamics of G protein-coupled receptors. J Biol Chem. 2007 Aug 24;282(34):25076-87. [Abstract] [Full Text]
  • *Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu M.M.: NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Mol Cell Biol. 2007 Apr;27(7):2527-37. [Abstract] [Full Text]
  • *Kreimann EL, Morales FC, de Orbeta-Cruz J, Takahashi Y, Adams H, Liu TJ, McCrea PD, Georgescu M.M.: Cortical stabilization of beta-catenin contributes to HERF1/EBP50 tumor suppressor function. Oncogene. 2007 Aug 9;26(36):5290-9. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

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