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Mouse Monoclonal to SIT

SIT-01 (IgG1)

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SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.


The antibody SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT.

Regulatory Status


Bacterially produced recombinant intracellular fragment of human SIT.

Species Reactivity:

  • Human

Negative Species:


  • Flow Cytometry
    Staining technique:
    intracellular staining
    Application note
  • Immunoprecipitation
  • Western Blotting
    Application note: Western blot analysis demonstrated that SIT is expressed as an approximately 40 kDa protein that is reduced to approximately 20 kDa by endoglycosidase treatment.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Simeoni L, Posevitz V, Kolsch U, Meinert I, Bruyns E, Pfeffer K, Reinhold D, Schraven B: The transmembrane adapter protein SIT regulates thymic development and peripheral T-cell functions. Mol Cell Biol. 2005 Sep;25(17):7557-68. [Abstract] [Full Text]
  • *Hubener C, Mincheva A, Lichter P, Schraven B, Bruyns E: Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2-interacting transmembrane adaptor protein (SIT). Immunogenetics. 2001 May-Jun;53(4):337-41. [Abstract]
  • *Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B: SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation. J Exp Med. 1999 Apr 19;189(8):1181-94. [Abstract] [Full Text]
  • Product Specific References

  • *Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
  • *Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    Fig. 1

    Fig. 1: Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01  and goat anti-mouse IgG (H+L)-HRP conjugate.

    SIT-01 PE

    Fig. 2: Intracellular staining of human peripheral blood using anti-SIT (clone SIT-01) PE.

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