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Mouse Monoclonal to Phosphotyrosine

P-Tyr-01 (IgG1)

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The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.

Regulatory Status


Phosphotyrosine conjugated to bovine serum albumin.

Species Reactivity:

  • Broad

Negative Species:


  • Western Blotting
    Recommended dilution:1-2 μg/ml
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Cebecauer M. et al., Biochem. Biophys. Res. Commun. 243, 706 (1998).
  • *Brdicka T. et al., Biochem. Biophys. Res. Commun. 248, 356 (1998).
  • Product Specific References

  • *Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B: Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604. [Abstract] [Full Text]
  • *Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178(4):2262-71. [Abstract] [Full Text]
  • *Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196(12):1617-26. [Abstract] [Full Text]
  • *Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    ICC P-Tyr-01

    Fig. 1. Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody-Alexa Fluor®  488 (green). Actin detected by phalloidin-Alexa Fluor®  567, DNA stained by DAPI (blue).


    Fig. 2. Flow cytometry detection (intracellular staining) of A431 cells after 30 min. stimulation with EGF, using anti-phosphotyrosine (P-Tyr-01) Alexa Fluor®  647.

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