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Mouse Monoclonal to CD326 / EpCAM

VU-1D9 (IgG1)

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Background

CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.

Specificity

The mouse monoclonal antibody VU-1D9 recognizes an epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

Regulatory Status

Immunogen

Small cell lung carcinoma cell line H69.

Species Reactivity:

  • Human
  • Other species Not tested

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 1-3 μg/ml
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 5-10 μg/ml
    Positive control: colon epithelium
    Application note: antigen retrieval: heat (sodium citrate) + trypsin; mAb incubation: 1 hour / RT; detection DAB
  • Immunoprecipitation
    Recommended dilution: 1-2 µg / 100-500 µg of protein in 1 ml lysate
  • Western Blotting
    Recommended dilution: 1-4 µg/ml
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
    Recommended dilution: 4 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Tsubura A, Senzaki H, Sasaki M, Hilgers J, Morii S: Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors. J Cutan Pathol. 1992 Feb;19(1):73-9. [Abstract]
  • *Ogura E, Senzaki H, Yoshizawa K, Hioki K, Tsubura A: Immunohistochemical localization of epithelial glycoprotein EGP-2 and carcinoembryonic antigen in normal colonic mucosa and colorectal tumors. Anticancer Res. 1998 Sep-Oct;18(5B):3669-75. [Abstract]
  • *Li G, Passebosc-Faure K, Lambert C, Gentil-Perret A, Blanc F, Oosterwijk E, Mosnier JF, Genin C, Tostain J: Flow cytometric analysis of antigen expression in malignant and normal renal cells. Anticancer Res. 2000 Jul-Aug;20(4):2773-8. [Abstract]
  • *Winter MJ, Nagtegaal ID, van Krieken JH, Litvinov SV: The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am J Pathol. 2003 Dec;163(6):2139-48. [Abstract] [Full Text]
  • *Brunner A, Prelog M, Verdorfer I, Tzankov A, Mikuz G, Ensinger C: EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 2008 Mar;61(3):307-10. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    VU-1D9 PerCP-Cy5-5


    Fig. 1. Surface staining of human MCF-7 cell line with anti-human CD326 / EpCAM (VU-1D9) PerCP-CyTM5.5.


    VU-1D9 purified


    Fig. 2. Surface staining of human MCF-7 cell line with anti-human CD326 / EpCAM (VU-1D9) purified.


    VU-1D9 PE-Cy7


    Fig. 3. Surface staining of human MCF-7 cell line with anti-human CD326 / EpCAM (VU-1D9) PE-CyTM7.

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