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Mouse Monoclonal to Sialyl Lewis a / CA-19.9

121SLE (IgM)

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  11-285-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-285-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Specificity

The antibody 121SLE recognizes Sialyl Lewis a containing glycolipids.

Regulatory Status

Immunogen

Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.

Species Reactivity:

  • Human
  • Other not tested

Negative Species:

Applications:

  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
    Positive control:colorectal adenocarcinoma
    Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Rouger Ph. et al. (Eds.): Proceedings of the First International Workshop on monoclonal antibodies against human red blood cells and related antigens (Paris, 1987) published in: Blood Transfusion and Immunohaematology 30 (5), pp 353-720.
  • *Rye PD, Bovin NV, Vlasova EV, Molodyk AA, Baryshnikov A, Kreutz FT, Garinther WI, Schultes BC, Noujaim AA, Madiyalakan R, Magnani J, Nilsson O, Nilsson K, Nustad K, Norum L, Bell H, Cao Y, Suresh MR, Very DL, Freeman JV, Yeung KK, Hilgers J: Summary report on the ISOBM TD-6 workshop: analysis of 20 monoclonal antibodies against Sialyl Lewisa and related antigens. Montreux, Switzerland, September 19-24, 1997. Tumour Biol. 1998;19(5):390-420. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgM Isotype Control
  • Example Data

    121SLE
    Fig. 1.


    Fig. 1. Immunohistochemistry staining of sialyl-Lewis a in colorectal adenocarcinoma (paraffin-embedded sections) with 121SLE antibody.

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