System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to IgE

4H10 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-326-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-326-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-326-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-326-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order


Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.


The antibody 4H10 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).

Regulatory Status


Purified human IgE.

Species Reactivity:

  • Human

Negative Species:


  • Flow Cytometry
    Recommended dilution:4 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95. [Abstract]
  • *Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71. [Abstract] [Full Text]
  • *Balogh Z, MerÚtey K, Falus A, Bozsˇky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34. [Abstract] [Full Text]
  • *Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32. [Abstract]
  • *Gould HJ, Beavil RL, Vercelli D: IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation. Br Med Bull. 2000;56(4):908-24. [Abstract] [Full Text]
  • Product Specific References

  • *НС Аляхнович, ВВ Янченко, ЕГ Асирян, ВП Мартинович, ВП Голубович: Выявление IgE-позитивных лейкоцитов крови синтетическим пептидом и моноклональными антителами. Immunopathology, allergology, infectology 2016, no.2, pages 28-34
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    4H10 FITC

    Fig. 1. Surface staining of IgE on human peripheral blood cells with anti-IgE (4H10) FITC.


    Fig. 2. Surface staining of IgE on human peripheral blood cells with anti-IgE (4H10) purified, GAM-APC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO┤s term and conditions which are available at
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy