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Mouse Monoclonal to IgE

BE5 (IgG1)

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  11-324-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1F-324-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-324-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-324-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-324-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  T8-324-C025 PE-Cy™5 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.

Specificity

The antibody BE5 reacts with human IgE; it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.

Regulatory Status

Immunogen

Purified human IgE.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 5 μg/ml
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95. [Abstract]
  • *Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71. [Abstract] [Full Text]
  • *Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34. [Abstract] [Full Text]
  • *Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32. [Abstract]
  • *Gould HJ, Beavil RL, Vercelli D: IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation. Br Med Bull. 2000;56(4):908-24. [Abstract] [Full Text]
  • Product Specific References

  • *Kaulfürst-Soboll H, Mertens M, Brehler R, von Schaewen A: Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis. PLoS One. 2011 Mar 14;6(3):e17800. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    BE5
    Fig. 1A Flow Cytometry analysis
    BE5
    Fig. 1B Flow Cytometry analysis



    Fig. 1. Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody.
    Combination of anti-human IgE (BE5) FITC and anti-human CD63 (MEM-259) PE-Dyomics 647 (cat. no. 1Y-343-T100) was used (analysis in basophil window).
    Fig. 1A - staining of non-stimulated (control) sample
    Fig. 1B - staining of IgE stimulated sample



    BE5 purified

    Fig. 2. Surface staining of IgE in human peripheral blood lymphocytes with anti-IgE (BE5) purified, GAM-APC.



    BE5 PE-Cy5

    Fig. 3. Surface staining of IgE in human peripheral blood with anti-IgE (BE5) PE-CyTM5.


    IgE BE5

    Fig. 4. Surface staining of IgE in human peripheral blood with anti-IgE (BE5) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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