Mouse Monoclonal to beta2-microglobulin
B2M-01 (IgG2a)
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Background
Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Specificity
The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Immunogen
Purified human beta2-microglobulin
Species Reactivity:
Negative Species:
- Mouse
- Bovine
- Canine (Dog)
- Rabbit
- Chicken
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Applications:
- Flow Cytometry
Recommended dilution: 1 μg/ml
Positive control: Peripheral blood lymphocytes
Negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes
- Immunoprecipitation
Application note: The B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
- Western Blotting
Recommended dilution: 2-4 μg/ml
Positive control:
U937 human histiocytic lymphoma cell line
RAJI human Burkitt lymphoma cell line
Negative control:
EL4 mouse lymphoblastic lymphoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
Application note: Both reducing and non-reducing conditions.
- Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: kidney glomeruli
- ELISA
Application note: Working dilution should be determinated by investigator.
- RIA
Application note: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
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Product Specific References
*Hilgert I, Horejsi V, Kristofova H.: The use of murine monoclonal antibody B2M-01 for detection and purification of
human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
[Abstract]
*Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD.: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep;21(9):1257-65. Epub 2006 Jun 30.
[Abstract] [Full Text]
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For research use only. Not for drug, diagnostic or other use.
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Mouse IgG2a Isotype Control
Example Data
Fig. 1. Western Blotting
Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (
B2M-01).
Lane 1: RAJI human Burkitt lymphoma cell line
Lane 2: EL4 mouse lymphoblastic lymphoma cell line
Lane 3: U937 human histiocytic lymphoma cell line
