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Mouse Monoclonal to beta2-Microglobulin

B2M-01 (IgG2a)

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  A6-237-C025 Alexa Fluor® 647 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Specificity

The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Regulatory Status

Immunogen

Purified human beta2-microglobulin

Species Reactivity:

  • Human

Negative Species:

  • Mouse
  • Bovine
  • Canine (Dog)
  • Rabbit
  • Chicken

Applications:

  • Flow Cytometry
    Recommended dilution: 1 μg/ml
    Positive control: Peripheral blood lymphocytes
    Negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes
  • Immunoprecipitation
    Application note: The B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
  • Western Blotting
    Recommended dilution: 2-4 μg/ml
    Positive control:
    U937 human histiocytic lymphoma cell line
    RAJI human Burkitt lymphoma cell line
    Negative control:
    EL4 mouse lymphoblastic lymphoma cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
    Application note: Both reducing and non-reducing conditions.
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Positive tissue: kidney glomeruli
  • Immunocytochemistry
  • ELISA
    Application note: Working dilution should be determinated by investigator.
  • RIA
    Application note: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76. [Abstract]
  • *Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep;21(9):1257-65. Epub 2006 Jun 30. [Abstract] [Full Text]
  • *Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79(24): 15226-15237. [Full Text]
  • *Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37(2):139-45. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2a Isotype Control
  • Example Data


    B2M-01


    Fig. 1: Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (B2M-01).
    Lane 1: RAJI human Burkitt lymphoma cell line
    Lane 2: EL4 mouse lymphoblastic lymphoma cell line
    Lane 3: U937 human histiocytic lymphoma cell line


    B2M ICC


    Fig. 2: Confocal microscopy of human β2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor® 647 conjugate  and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control (A6-458-C100). Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2µg/ml. 



    B2M-01 FITC


    Fig. 3. Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) FITC.


    B2M-01 PE


    Fig. 4. Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PE.


    B2M-01 PerCP-Cy5-5


    Fig. 5. Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PerCP-CyTM5.5.


    B2M-01 biotin


    Fig. 6. Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) biotin.


    B2M-01 HRP


    Fig. 7. ELISA analysis of HLA-G and β2-microglobulin in supernatant of HLA-G transfectants with anti-β2-microglobulin (B2M-01) HRP as the detection antibody.


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