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Mouse Monoclonal to HLA-Class I

MEM-123 (IgG3)

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  11-363-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1P-363-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Specificity

The antibody MEM-123 reacts with all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types.
The antibody MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9.

Regulatory Status

Immunogen

COS-7 African green monkey kidney cells

Species Reactivity:

  • Human
  • Non-Human Primates
  • Bovine

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:4 μg/ml
  • Immunoprecipitation
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

References

  • Unpublished.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG3 Isotype Control
  • Example Data


    MEM-123 pur


    Fig. 1. Surface staining of human peripheral blood with anti-HLA-class I (MEM-123) purified, GAM-APC.


    MEM-123 PE


    Fig. 2. Surface staining of K562 cells with anti-HLA-class I (MEM-123) PE.

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