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Mouse Monoclonal to HLA-G

MEM-G/1 (IgG1)

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  11-291-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-291-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  11-291-M001 purified 1.0 mg yes choose region PDF datasheetHTML datasheet buy
  1B-291-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-291-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Specificity

The antibody MEM-G/1 reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.

Regulatory Status

Immunogen

Denatured bacterially expressed recombinant human HLA-G heavy chain.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 1:60 to 1:100
    Incubation: 1 h at RT
    Positive tissue: Human placenta - extravillous cytotrophoblast
    Staining technique: Standard ABC technique
    Pretreatment: Heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven)
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Fournel S, Huc X, Aguerre-Girr M, Solier C, Legros M, Praud-Brethenou C, Moussa M, Chaouat G, Berrebi A, Bensussan A, Lenfant F, Le Bouteiller P: Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens. 2000 Jun;55(6):510-8. [Abstract]
  • *Frumento G, Franchello S, Palmisano GL, Nicotra MR, Giacomini P, Loke YW, Geraghty DE, Maio M, Manzo C, Natali PG, Ferrara GB: Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment. Tissue Antigens. 2000 Jul;56(1):30-7. [Abstract]
  • *Hurks HM, Valter MM, Wilson L, Hilgert I, van den Elsen PJ, Jager MJ: Uveal melanoma: no expression of HLA-G. Invest Ophthalmol Vis Sci. 2001 Dec;42(13):3081-4. [Abstract] [Full Text]
  • *Boyson JE, Erskine R, Whitman MC, Chiu M, Lau JM, Koopman LA, Valter MM, Angelisova P, Horejsi V, Strominger JL: Disulfide bond-mediated dimerization of HLA-G on the cell surface. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16180-5. Epub 2002 Nov 26. [Abstract] [Full Text]
  • *Shih IM, Kurman RJ: p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations. Am J Surg Pathol. 2004 Sep;28(9):1177-83. [Abstract]
  • *Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules. J Immunol. 2005 Oct 15;175(8):4866-74. [Abstract] [Full Text]
  • *Amiot L, Vu N, Rauch M, Helgoualc'h AL, Chalmel F, Gascan H, Turlin B, Guyader D, Samson M: Expression of HLA-G by mast cells is associated with hepatitis C virus-induced liver fibrosis. J Hepatol. 2013 Sep 11. pii: S0168-8278(13) [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    HLA-G staining in infiltrating macrophages
    Fig. 1A Immunohistochemistry (pulmonary disseases)
    MEM-G/1 placenta
    Fig. 1B Immunohistochemistry (first-trimester placenta)

    Fig. 1. Immunohistochemistry staining with anti-human HLA-G (MEM-G/1).
    Fig. 1A - pulmonary disseases (paraffin-embedded sections) The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.
    Fig. 1B - first-trimester placenta (paraffin-embedded sections)

    MEM-G-1 biotin

    Fig. 2. Western blotting analysis (reducing conditions) of HLA-G1 in HLA-G1 transfectants using the antibody MEM-G/1 biotin.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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