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Mouse Monoclonal to HLA-G

MEM-G/1 (IgG1)

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The antibody MEM-G/1 reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.

Regulatory Status


Denatured bacterially expressed recombinant human HLA-G heavy chain.

Species Reactivity:

  • Human

Negative Species:


  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 1:60 to 1:100
    Incubation: 1 h at RT
    Positive tissue: Human placenta - extravillous cytotrophoblast
    Staining technique: Standard ABC technique
    Pretreatment: Heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven)
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Fournel S, Huc X, Aguerre-Girr M, Solier C, Legros M, Praud-Brethenou C, Moussa M, Chaouat G, Berrebi A, Bensussan A, Lenfant F, Le Bouteiller P: Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens. 2000 Jun;55(6):510-8. [Abstract]
  • *Frumento G, Franchello S, Palmisano GL, Nicotra MR, Giacomini P, Loke YW, Geraghty DE, Maio M, Manzo C, Natali PG, Ferrara GB: Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment. Tissue Antigens. 2000 Jul;56(1):30-7. [Abstract]
  • *Hurks HM, Valter MM, Wilson L, Hilgert I, van den Elsen PJ, Jager MJ: Uveal melanoma: no expression of HLA-G. Invest Ophthalmol Vis Sci. 2001 Dec;42(13):3081-4. [Abstract] [Full Text]
  • *Boyson JE, Erskine R, Whitman MC, Chiu M, Lau JM, Koopman LA, Valter MM, Angelisova P, Horejsi V, Strominger JL: Disulfide bond-mediated dimerization of HLA-G on the cell surface. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16180-5. Epub 2002 Nov 26. [Abstract] [Full Text]
  • *Shih IM, Kurman RJ: p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations. Am J Surg Pathol. 2004 Sep;28(9):1177-83. [Abstract]
  • *Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules. J Immunol. 2005 Oct 15;175(8):4866-74. [Abstract] [Full Text]
  • *Amiot L, Vu N, Rauch M, Helgoualc'h AL, Chalmel F, Gascan H, Turlin B, Guyader D, Samson M: Expression of HLA-G by mast cells is associated with hepatitis C virus-induced liver fibrosis. J Hepatol. 2013 Sep 11. pii: S0168-8278(13) [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    HLA-G staining in infiltrating macrophages
    Fig. 1A Immunohistochemistry (pulmonary disseases)
    MEM-G/1 placenta
    Fig. 1B Immunohistochemistry (first-trimester placenta)

    Fig. 1. Immunohistochemistry staining with anti-human HLA-G (MEM-G/1).
    Fig. 1A - pulmonary disseases (paraffin-embedded sections) The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.
    Fig. 1B - first-trimester placenta (paraffin-embedded sections)

    MEM-G-1 biotin

    Fig. 2. Western blotting analysis (reducing conditions) of HLA-G1 in HLA-G1 transfectants using the antibody MEM-G/1 biotin.

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