System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to HLA-G

MEM-G/9 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-292-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-292-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  11-292-M001 purified 1.0 mg yes choose region PDF datasheetHTML datasheet buy
  10-292-C025 azide free 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  10-292-C100 azide free 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-292-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-292-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-292-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-292-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-292-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-292-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1A-292-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1A-292-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

Specificity

The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported.
The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).

Regulatory Status

Immunogen

Recombinant human HLA-G refolded with beta2-microglobulin and peptide.

Species Reactivity:

  • Human

Negative Species:

  • Mouse

Applications:

  • Flow Cytometry
    Recommended dilution: 1-5 μg/ml
    Positive control: JEG-3 human choriocarcinoma cell line
  • Immunoprecipitation
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
    Recommended dilution:
    Alexa Fluor® 488 Fab-fragment: 5 μg/ml
  • ELISA
    Application note: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32.
    Coating antibody (10 μg/ml)
    Detection antibody (biotin or peroxidase conjugate; 1 μg/ml)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Fournel S, Huc X, Aguerre-Girr M, Solier C, Legros M, Praud-Brethenou C, Moussa M, Chaouat G, Berrebi A, Bensussan A, Lenfant F, Le Bouteiller P: Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens. 2000 Jun;55(6):510-8. [Abstract]
  • *Lozano JM, Gonzalez R, Kindelan JM, Rouas-Freiss N, Caballos R, Dausset J, Carosella ED, Pena J: Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule. AIDS. 2002 Feb 15;16(3):347-51. [Abstract]
  • *Pangault C, Le Friec G, Caulet-Maugendre S, Lena H, Amiot L, Guilloux V, Onno M, Fauchet R: Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases. Hum Immunol. 2002 Feb;63(2):83-90. [Abstract]
  • *Fuzzi B, Rizzo R, Criscuoli L, Noci I, Melchiorri L, Scarselli B, Bencini E, Menicucci A, Baricordi OR: HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. Eur J Immunol. 2002 Feb;32(2):311-5. [Abstract]
  • *Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26. [Abstract]
  • *Abstracts from the 3rd International Conference on HLA-G. Tissue Antigens 62, 339-357 (2003).
  • *Lopez AS, Alegre E, LeMaoult J, Carosella E, Gonzalez A. Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells. Mol Immunol. 2006 Jul;43(14):2151-60. [Abstract]
  • *Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules. J Immunol. 2005 Oct 15;175(8):4866-74. [Abstract] [Full Text]
  • *Zhao L, Teklemariam T, Hantash BM: Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies. Tissue Antigens. 2012 Sep;80(3):231-8 [Abstract]
  • *And other.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MEM-G/9


    Fig. 1. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC (cat. no. 1F-389-C100).


    FabMEMG9


    Fig. 2. Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-human HLA-G (MEM-G/9) Alexa Fluor® 488 Fab-fragment.



    WBMEMG9


    Fig. 3. Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G (4H84; cat. no. 11-494-C100) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).


    MEM-G-9 FITC


    Fig. 4. Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC.


    MEM-G-9 PE


    Fig. 5. Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.


    MEM-G-9 biotin


    Fig. 6. Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) biotin, streptavidin/GAM-APC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy