categories: MHC (Major Histocompatibility Complex) and Related Antigens (Human)

Mouse Monoclonal to HLA-G

MEM-G/9 (IgG1)

Product No.	Form	Quantity	Price
11-292-C025	purified	0.025 mg	choose region
11-292-C100	purified	0.1 mg	choose region
11-292-M001	purified	1.0 mg	choose region
10-292-C025	azide free	0.025 mg	choose region
10-292-C100	azide free	0.1 mg	choose region
1X-292-C025	Peroxidase	0.025 mg	choose region
1X-292-C100	Peroxidase	0.1 mg	choose region
1B-292-C025	biotin	0.025 mg	choose region
1B-292-C100	biotin	0.1 mg	choose region
1F-292-C025	FITC	0.025 mg	choose region
1F-292-C100	FITC	0.1 mg	choose region
A4-292-C025	Alexa Fluor® 488	0.025 mg	choose region
A4-292-C100	Alexa Fluor® 488	0.1 mg	choose region
F4-292-C050	Alexa Fluor® 488 Fab fragment	0.05 mg	choose region
1P-292-C025	PE	0.025 mg	choose region
1P-292-C100	PE	0.1 mg	choose region
1A-292-C025	APC	0.025 mg	choose region
1A-292-C100	APC	0.1 mg	choose region
A6-292-C025	Alexa Fluor® 647	0.025 mg	choose region
A6-292-C100	Alexa Fluor® 647	0.1 mg	choose region

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- Manufacture on order

Background

Entrez Gene: 6p21.3

Specificity

The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).

Immunogen

Recombinant human HLA-G refolded with beta2-microglobulin and peptide.

Species Reactivity: Human Negative Species: Mouse	Applications: Flow Cytometry Recommended dilution: 1-5 μg/ml Positive control: JEG-3 human choriocarcinoma cell line Immunoprecipitation Immunohistochemistry (frozen sections) Immunocytochemistry Recommended dilution: Alexa Fluor® 488 Fab-fragment: 5 μg/ml ELISA Application note: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody (10 μg/ml) Detection antibody (biotin or peroxidase conjugate; 1 μg/ml) Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Fournel S, Huc X, Aguerre-Girr M, Solier C, Legros M, Praud-Brethenou C, Moussa M, Chaouat G, Berrebi A, Bensussan A, Lenfant F, Le Bouteiller P.: Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens. 2000 Jun;55(6):510-8. [Abstract] Lozano JM, Gonzalez R, Kindelan JM, Rouas-Freiss N, Caballos R, Dausset J, Carosella ED, Pena J.: Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule. AIDS. 2002 Feb 15;16(3):347-51. [Abstract] Pangault C, Le Friec G, Caulet-Maugendre S, Lena H, Amiot L, Guilloux V, Onno M, Fauchet R.: Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases. Hum Immunol. 2002 Feb;63(2):83-90. [Abstract] Fuzzi B, Rizzo R, Criscuoli L, Noci I, Melchiorri L, Scarselli B, Bencini E, Menicucci A, Baricordi OR.: HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. Eur J Immunol. 2002 Feb;32(2):311-5. [Abstract] Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N.: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26. [Abstract] Abstracts from the 3rd International Conference on HLA-G. Tissue Antigens 62, 339-357 (2003). Lopez AS, Alegre E, LeMaoult J, Carosella E, Gonzalez A. Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells. Mol Immunol. 2006 Jul;43(14):2151-60. [Abstract] Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules. J Immunol. 2005 Oct 15;175(8):4866-74. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Mouse

Applications:

Flow Cytometry
Recommended dilution: 1-5 μg/ml
Positive control: JEG-3 human choriocarcinoma cell line
Immunoprecipitation
Immunohistochemistry (frozen sections)
Immunocytochemistry
Recommended dilution:
Alexa Fluor® 488 Fab-fragment: 5 μg/ml
ELISA
Application note: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32.
Coating antibody (10 μg/ml)
Detection antibody (biotin or peroxidase conjugate; 1 μg/ml)

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Fournel S, Huc X, Aguerre-Girr M, Solier C, Legros M, Praud-Brethenou C, Moussa M, Chaouat G, Berrebi A, Bensussan A, Lenfant F, Le Bouteiller P.: Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens. 2000 Jun;55(6):510-8. [Abstract]

*Lozano JM, Gonzalez R, Kindelan JM, Rouas-Freiss N, Caballos R, Dausset J, Carosella ED, Pena J.: Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule. AIDS. 2002 Feb 15;16(3):347-51. [Abstract]

*Pangault C, Le Friec G, Caulet-Maugendre S, Lena H, Amiot L, Guilloux V, Onno M, Fauchet R.: Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases. Hum Immunol. 2002 Feb;63(2):83-90. [Abstract]

*Fuzzi B, Rizzo R, Criscuoli L, Noci I, Melchiorri L, Scarselli B, Bencini E, Menicucci A, Baricordi OR.: HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. Eur J Immunol. 2002 Feb;32(2):311-5. [Abstract]

*Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N.: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26. [Abstract]

*Abstracts from the 3rd International Conference on HLA-G. Tissue Antigens 62, 339-357 (2003).

*Lopez AS, Alegre E, LeMaoult J, Carosella E, Gonzalez A. Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells. Mol Immunol. 2006 Jul;43(14):2151-60. [Abstract]

*Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules. J Immunol. 2005 Oct 15;175(8):4866-74. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG1 Isotype Control

Example Data

Fig. 1.

Fig. 1. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC (cat. no. 1F-389-C100).

Fig. 2.

Fig. 2. Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-human HLA-G (MEM-G/9) Alexa Fluor^® 488 Fab-fragment.

Fig. 3.

Fig. 3. Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G (4H84; cat. no. 11-494-C100) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

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