categories: MHC (Major Histocompatibility Complex) and Related Antigens (Human)

Mouse Monoclonal to HLA-DR+DP

MEM-136 (IgG1)

Product No.	Form	Quantity	Price
11-296-C025	purified	0.025 mg	choose region
11-296-C100	purified	0.1 mg	choose region
1B-296-C025	biotin	0.025 mg	choose region
1B-296-C100	biotin	0.1 mg	choose region
PB-296-T025	Pacific Blue™	25 tests	choose region
PB-296-T100	Pacific Blue™	100 tests	choose region
1F-296-T025	FITC	25 tests	choose region
1F-296-T100	FITC	100 tests	choose region
1P-296-T025	PE	25 tests	choose region
1P-296-T100	PE	100 tests	choose region
1A-296-T025	APC	25 tests	choose region
1A-296-T100	APC	100 tests	choose region
A7-296-T025	Alexa Fluor® 700	25 tests	choose region
A7-296-T100	Alexa Fluor® 700	100 tests	choose region

- In stock! Ready for immediate despatch

- Manufacture on order

Background

Gene Locus: 6p21.3

Specificity

The antibody MEM-136 recognizes common epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).

Immunogen

PHA-activated peripheral blood lymphocytes.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution:0.5 μg/ml Immunoprecipitation Western Blotting Application note: Non-reducing conditions. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References *Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H: T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May;11(5):777-86. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:0.5 μg/ml
Immunoprecipitation
Western Blotting
Application note: Non-reducing conditions.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H: T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May;11(5):777-86. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG1 Isotype Control

Example Data

Fig. 1. Western Blotting

Fig. 1. Western Blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136).
The antibody MEM-136 recognizes β-chain of HLA-DR antigen under non-reducing conditions.
Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136)
Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; 11-457-C100)

Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)

DyLight™ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Cy5, Cy5.5 and Cy7 conjugates are made and sold under license from GE Healthcare Bio-Sciences Corp. The conjugates are licensed for non-commercial research use only. Not for therapeutic or in vivo applications. Other use needs license from GE Healthcare Bio-Sciences Corp. under U.S. patent Nos. 5 569 587 and 5 627 027 and other patents pending.

PRODUCTS