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Mouse Monoclonal to HLA-DR+DP

MEM-136 (IgG1)

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Background

Specificity

The antibody MEM-136 recognizes common epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).

Regulatory Status

Immunogen

PHA-activated peripheral blood lymphocytes.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:0.5 μg/ml
  • Immunoprecipitation
  • Western Blotting
    Application note: Non-reducing conditions.
  • Immunohistochemistry (paraffin sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H: T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May;11(5):777-86. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MEM-136



    Fig. 1. Western Blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136).
    The antibody MEM-136 recognizes β-chain of HLA-DR antigen under non-reducing conditions.
    Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136)
    Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; 11-457-C100)

    MEM-136 Pacific Blue FC


    Fig. 2. Surface staining of human peripheral blood with anti-human HLA-DR+DP (MEM-136) Pacific BlueTM.


    MEM-136 biotin


    Fig. 3. Surface staining of human peripheral blood with anti-human HLA-DR+DP (MEM-136) biotin.


    mem-136 APC


    Fig. 4. Surface staining of human peripheral blood with anti-human HLA-DR+DP (MEM-136) APC.




    MEM-136 PE


    Fig. 5. Surface staining of human peripheral blood with anti-human HLA-DR+DP (MEM-136) PE.


    LS 296


    Fig. 6. Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-HLA-DR+DP (MEM-136).
    Commercially tested by LifeSpan BioSciences.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
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    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
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