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Mouse Monoclonal to CD222 / IGF2 receptor / CIMPR

MEM-238 (IgG1)

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CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.


The antibody MEM-238 recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.

Regulatory Status


Recombinant Vaccinia virus encoding CD222.

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:


  • Flow Cytometry
    Recommended dilution:1-2 μg/ml
  • Western Blotting
    Application note: Non-reducing conditions.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).
  • *Gasanov U, Koina C, Beagley KW, Aitken RJ, Hansbro PM: Identification of the insulin-like growth factor II receptor as a novel receptor for binding and invasion by Listeria monocytogenes. Infect Immun. 2006 Jan;74(1):566-77. [Abstract] [Full Text]
  • *Wood RJ, Hulett MD: Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation. J Biol Chem. 2008 Feb 15;283(7):4165-76. [Abstract]
  • Product Specific References

  • *Leksa V, Godar S, Cebecauer M, Hilgert I, Breuss J, Weidle UH, Horejsi V, Binder BR, Stockinger H: The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem. 2002 Oct 25;277(43):40575-82. [Abstract] [Full Text]
  • *Schatzlmaier P, Supper V, G÷schl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11 [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    Western Blotting

    Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line.
    Lane 1: immunostaining with anti-CD222 (MEM-238)
    Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)

    MEM-238 PE FC

    Fig. 2. Intracellular staining of human peripheral blood with anti-CD222 (MEM-238) PE.

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