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Mouse Monoclonal to CD162 / PSGL-1

TB5 (IgG1)

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  11-312-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.

Specificity

The antibody TB5 reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells.

Regulatory Status

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunohistochemistry (paraffin sections)
    Positive tissue:tonsil, spleen
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Moore KL: Structure and function of P-selectin glycoprotein ligand-1. Leuk Lymphoma. 1998 Mar;29(1-2):1-15. [Abstract]
  • *Lévesque JP, Zannettino AC, Pudney M, Niutta S, Haylock DN, Snapp KR, Kansas GS, Berndt MC, Simmons PJ. PSGL-1-mediated adhesion of human hematopoietic progenitors to P-selectin results in suppression of hematopoiesis. Immunity. 1999 Sep;11(3):369-78. [Abstract]
  • *Davenpeck KL, Brummet ME, Hudson SA, Mayer RJ, Bochner BS: Activation of human leukocytes reduces surface P-selectin glycoprotein ligand-1 (PSGL-1, CD162) and adhesion to P-selectin in vitro. J Immunol. 2000 Sep 1;165(5):2764-72. [Abstract] [Full Text]
  • *Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).
  • *Marsik C, Mayr F, Cardona F, Schaller G, Wagner OF, Jilma B: Endotoxin down-modulates P-selectin glycoprotein ligand-1 (PSGL-1, CD162) on neutrophils in humans. J Clin Immunol. 2004 Jan;24(1):62-5. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

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