categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD105 / Endoglin

MEM-226 (IgG2a)

Product No.	Form	Quantity	Price
11-298-C025	purified	0.025 mg	choose region
11-298-C100	purified	0.1 mg	choose region
PB-298-T025	Pacific Blue™	25 tests	choose region
PB-298-T100	Pacific Blue™	100 tests	choose region
1F-298-T025	FITC	25 tests	choose region
1F-298-T100	FITC	100 tests	choose region
A4-298-T025	Alexa Fluor® 488	25 tests	choose region
A4-298-T100	Alexa Fluor® 488	100 tests	choose region
1P-298-T025	PE	25 tests	choose region
1P-298-T100	PE	100 tests	choose region
1A-298-T025	APC	25 tests	choose region
1A-298-T100	APC	100 tests	choose region
A6-298-T025	Alexa Fluor® 647	25 tests	choose region
A6-298-T100	Alexa Fluor® 647	100 tests	choose region
1D-298-T025	Dyomics 647	25 tests	choose region
1D-298-T100	Dyomics 647	100 tests	choose region
A7-298-T025	Alexa Fluor® 700	25 tests	choose region
A7-298-T100	Alexa Fluor® 700	100 tests	choose region

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Background

CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Entrez Gene: 9q33-q34.1

Specificity

The antibody MEM-226 reacts with CD105 (Endoglin), a 180 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Immunogen

Recombinant Vaccinia virus containing the human CD105 cDNA.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Immunoprecipitation Western Blotting Application note: Non-reducing conditions. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Zhu Y, Sun Y, Xie L, Jin K, Sheibani N, Greenberg DA: Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells. Stroke. 2003 Oct;34(10):2483-8. [Abstract] [Full Text] Li C, Issa R, Kumar P, Hampson IN, Lopez-Novoa JM, Bernabeu C, Kumar S: CD105 prevents apoptosis in hypoxic endothelial cells. J Cell Sci. 2003 Jul 1;116(Pt 13):2677-85. [Abstract] [Full Text] Guo B, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C, Kumar S: CD105 inhibits transforming growth factor-beta-Smad3 signalling. Anticancer Res. 2004 May-Jun;24(3a):1337-45. [Abstract] Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25(3B):1851-64. [Abstract] *Piao M, Tokunaga O: Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb. 2006 Apr;13(2):82-9. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
Western Blotting
Application note: Non-reducing conditions.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Zhu Y, Sun Y, Xie L, Jin K, Sheibani N, Greenberg DA: Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells. Stroke. 2003 Oct;34(10):2483-8. [Abstract] [Full Text]

*Li C, Issa R, Kumar P, Hampson IN, Lopez-Novoa JM, Bernabeu C, Kumar S: CD105 prevents apoptosis in hypoxic endothelial cells. J Cell Sci. 2003 Jul 1;116(Pt 13):2677-85. [Abstract] [Full Text]

*Guo B, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C, Kumar S: CD105 inhibits transforming growth factor-beta-Smad3 signalling. Anticancer Res. 2004 May-Jun;24(3a):1337-45. [Abstract]

*Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25(3B):1851-64. [Abstract]

*Piao M, Tokunaga O: Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb. 2006 Apr;13(2):82-9. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG2a Isotype Control

Mouse Monoclonal to CD105 MEM-229 (IgG2a)

Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Surface staining of HUVEC (human umbilical vein endothelial cells) with anti-human CD105 (MEM-226) FITC.
Total viable cells were used for analysis.

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