categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD99R

MEM-131 (IgM)

Product No.	Form	Quantity	Price
11-236-C025	purified	0.025 mg	choose region
11-236-C100	purified	0.1 mg	choose region
1F-236-T025	FITC	25 tests	choose region
1F-236-T100	FITC	100 tests	choose region
1P-236-T025	PE	25 tests	choose region
1P-236-T100	PE	100 tests	choose region

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Background

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Entrez Gene: Xp22.32; Yp11.3

Specificity

The antibody MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes.
HLDA V; WS Code AS S020
HLDA V; WS Code T T-E2.02
HLDA V; WS Code T T-017

Immunogen

HPB-ALL human peripheral blood leukemia T-cell line

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution: 1 μg/ml Immunoprecipitation Western Blotting Application note: Non-reducing conditions. Immunohistochemistry (paraffin sections) Recommended dilution: 10 μg/ml Positive tissue: thymic lymphocytes Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Gelin C, Aubrit F, Phalipon A, Raynal B, Cole S, Kaczorek M, Bernard A: The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO J. 1989 Nov;8(11):3253-9. [Abstract] [Full Text] Bernard G, Zoccola D, Deckert M, Breittmayer JP, Aussel C, Bernard A: The E2 molecule (CD99) specifically triggers homotypic aggregation of CD4+ CD8+ thymocytes. J Immunol. 1995 Jan 1;154(1):26-32. [Abstract] Bernard G, Breittmayer JP, de Matteis M, Trampont P, Hofman P, Senik A, Bernard A: Apoptosis of immature thymocytes mediated by E2/CD99. J Immunol. 1997 Mar 15;158(6):2543-50. [Abstract] Bernard G, Raimondi V, Alberti I, Pourtein M, Widjenes J, Ticchioni M, Bernard A: CD99 (E2) up-regulates alpha4beta1-dependent T cell adhesion to inflamed vascular endothelium under flow conditions. Eur J Immunol. 2000 Oct;30(10):3061-5. [Abstract] Product Specific References Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61. [Abstract] [Full Text] *Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85(9):2402-13. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 1 μg/ml
Immunoprecipitation
Western Blotting
Application note: Non-reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: thymic lymphocytes

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Gelin C, Aubrit F, Phalipon A, Raynal B, Cole S, Kaczorek M, Bernard A: The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO J. 1989 Nov;8(11):3253-9. [Abstract] [Full Text]

*Bernard G, Zoccola D, Deckert M, Breittmayer JP, Aussel C, Bernard A: The E2 molecule (CD99) specifically triggers homotypic aggregation of CD4+ CD8+ thymocytes. J Immunol. 1995 Jan 1;154(1):26-32. [Abstract]

*Bernard G, Breittmayer JP, de Matteis M, Trampont P, Hofman P, Senik A, Bernard A: Apoptosis of immature thymocytes mediated by E2/CD99. J Immunol. 1997 Mar 15;158(6):2543-50. [Abstract]

*Bernard G, Raimondi V, Alberti I, Pourtein M, Widjenes J, Ticchioni M, Bernard A: CD99 (E2) up-regulates alpha4beta1-dependent T cell adhesion to inflamed vascular endothelium under flow conditions. Eur J Immunol. 2000 Oct;30(10):3061-5. [Abstract]

Product Specific References

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

*Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61. [Abstract] [Full Text]

*Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85(9):2402-13. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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