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Mouse Monoclonal to CD99R

MEM-131 (IgM)

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Background

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Specificity

The antibody MEM-131 reacts with CD99R, an epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes.
HLDA V; WS Code AS S020
HLDA V; WS Code T T-E2.02
HLDA V; WS Code T T-017

Regulatory Status

Immunogen

HPB-ALL human peripheral blood leukemia T-cell line

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 1 μg/ml
  • Immunoprecipitation
  • Western Blotting
    Application note: Non-reducing conditions.
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Positive tissue: thymic lymphocytes
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Gelin C, Aubrit F, Phalipon A, Raynal B, Cole S, Kaczorek M, Bernard A: The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO J. 1989 Nov;8(11):3253-9. [Abstract] [Full Text]
  • *Bernard G, Zoccola D, Deckert M, Breittmayer JP, Aussel C, Bernard A: The E2 molecule (CD99) specifically triggers homotypic aggregation of CD4+ CD8+ thymocytes. J Immunol. 1995 Jan 1;154(1):26-32. [Abstract]
  • *Bernard G, Breittmayer JP, de Matteis M, Trampont P, Hofman P, Senik A, Bernard A: Apoptosis of immature thymocytes mediated by E2/CD99. J Immunol. 1997 Mar 15;158(6):2543-50. [Abstract]
  • *Bernard G, Raimondi V, Alberti I, Pourtein M, Widjenes J, Ticchioni M, Bernard A: CD99 (E2) up-regulates alpha4beta1-dependent T cell adhesion to inflamed vascular endothelium under flow conditions. Eur J Immunol. 2000 Oct;30(10):3061-5. [Abstract]
  • Product Specific References

  • *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  • *Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61. [Abstract] [Full Text]
  • *Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85(9):2402-13. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

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