categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD72

3F3 (IgG2b)

Product No.	Form	Quantity	Price
11-310-C025	purified	0.025 mg	choose region
11-310-C100	purified	0.1 mg	choose region
1B-310-C025	biotin	0.025 mg	choose region
1B-310-C100	biotin	0.1 mg	choose region
1P-310-T025	PE	25 tests	choose region
1P-310-T100	PE	100 tests	choose region

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Background

CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.

Entrez Gene: 9p13.3

Specificity

The antibody 3F3 reacts with CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells.
HLDA V; WS Code B CD72.5
HLDA VI; WS Code B CD72.1
HLDA VI; WS Code 6 BP 84

Immunogen

Normal human lymphocytes from a lymph node.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution:1 μg/ml Immunoprecipitation Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Kumanogoh A, Watanabe C, Lee I, Wang X, Shi W, Araki H, Hirata H, Iwahori K, Uchida J, Yasui T, Matsumoto M, Yoshida K, Yakura H, Pan C, Parnes JR, Kikutani H: Identification of CD72 as a lymphocyte receptor for the class IV semaphorin CD100: a novel mechanism for regulating B cell signaling. Immunity. 2000 Nov;13(5):621-31. [Abstract] Kumanogoh A, Kikutani H: The CD100-CD72 interaction: a novel mechanism of immune regulation. Trends Immunol. 2001 Dec;22(12):670-6. [Abstract] Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H. Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment. Int Immunol. 2005 Oct;17(10):1277-82. [Abstract] [Full Text] Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS ONE. 2007 Sep 5;2(9):e818. [Abstract] [Full Text] Product Specific References Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS One. 2007 Sep 5;2(9):e818. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:1 μg/ml
Immunoprecipitation

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Kumanogoh A, Watanabe C, Lee I, Wang X, Shi W, Araki H, Hirata H, Iwahori K, Uchida J, Yasui T, Matsumoto M, Yoshida K, Yakura H, Pan C, Parnes JR, Kikutani H: Identification of CD72 as a lymphocyte receptor for the class IV semaphorin CD100: a novel mechanism for regulating B cell signaling. Immunity. 2000 Nov;13(5):621-31. [Abstract]

*Kumanogoh A, Kikutani H: The CD100-CD72 interaction: a novel mechanism of immune regulation. Trends Immunol. 2001 Dec;22(12):670-6. [Abstract]

*Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H. Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment. Int Immunol. 2005 Oct;17(10):1277-82. [Abstract] [Full Text]

*Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS ONE. 2007 Sep 5;2(9):e818. [Abstract] [Full Text]

Product Specific References

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS One. 2007 Sep 5;2(9):e818. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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