categories: CD and Related Antigens (Human), Endocytosis (Human)

Mouse Monoclonal to CD71 / Transferrin Receptor

MEM-75 (IgG1)

Product No.	Form	Quantity	Price
11-235-C025	purified	0.025 mg	choose region
11-235-C100	purified	0.1 mg	choose region
1B-235-C025	biotin	0.025 mg	choose region
1B-235-C100	biotin	0.1 mg	choose region
PB-235-T025	Pacific Blue™	25 tests	choose region
PB-235-T100	Pacific Blue™	100 tests	choose region
1F-235-T025	FITC	25 tests	choose region
1F-235-T100	FITC	100 tests	choose region
A4-235-T025	Alexa Fluor® 488	25 tests	choose region
A4-235-T100	Alexa Fluor® 488	100 tests	choose region
1P-235-T025	PE	25 tests	choose region
1P-235-T100	PE	100 tests	choose region
1T-235-T025	PE-Dyomics 590	25 tests	choose region
1T-235-T100	PE-Dyomics 590	100 tests	choose region
1Y-235-T025	PE-Dyomics 647	25 tests	choose region
1Y-235-T100	PE-Dyomics 647	100 tests	choose region
1A-235-T025	APC	25 tests	choose region
1A-235-T100	APC	100 tests	choose region
A6-235-T025	Alexa Fluor® 647	25 tests	choose region
A6-235-T100	Alexa Fluor® 647	100 tests	choose region
A7-235-T025	Alexa Fluor® 700	25 tests	choose region
A7-235-T100	Alexa Fluor® 700	100 tests	choose region

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Background

CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.

Gene Locus: 3q29

Specificity

The antibody MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes.
The antibody MEM-75 does not block binding of transferrin to the receptor.
HLDA IV; WS Code A 45
HLDA V; WS Code T T-165

Immunogen

NALM-6 human pre-B cell line

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Immunoprecipitation Immunocytochemistry Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1(3):E79. [Abstract] [Full Text] Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205(4):297-318. [Abstract] [Full Text] Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13(35):4725-36. [Abstract] Graham RM, Reutens GM, Herbison CE, Delima RD, Chua AC, Olynyk JK, Trinder D: Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron. J Hepatol. 2008 Feb;48(2):327-34. [Abstract] Product Specific References Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
Immunocytochemistry

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1(3):E79. [Abstract] [Full Text]

*Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205(4):297-318. [Abstract] [Full Text]

*Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13(35):4725-36. [Abstract]

*Graham RM, Reutens GM, Herbison CE, Delima RD, Chua AC, Olynyk JK, Trinder D: Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron. J Hepatol. 2008 Feb;48(2):327-34. [Abstract]

Product Specific References

*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

For research use only. Not for drug, diagnostic or other use.

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Example Data

Fig. 1.

Fig. 1. Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75) biotin; detection by Streptavidin-PE (cat. no. SAVP-C500).
Total viable cells were used for analysis.

Fig. 2.

Fig. 2. Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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