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Mouse Monoclonal to CD71 / Transferrin Receptor

MEM-75 (IgG1)

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Background

CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.

Specificity

The antibody MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes.
The antibody MEM-75 does not block binding of transferrin to the receptor.
HLDA IV; WS Code A 45
HLDA V; WS Code T T-165

Regulatory Status

Immunogen

NALM-6 human pre-B cell line

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:4 μg/ml
  • Immunoprecipitation
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1(3):E79. [Abstract] [Full Text]
  • *Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205(4):297-318. [Abstract] [Full Text]
  • *Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13(35):4725-36. [Abstract]
  • *Graham RM, Reutens GM, Herbison CE, Delima RD, Chua AC, Olynyk JK, Trinder D: Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron. J Hepatol. 2008 Feb;48(2):327-34. [Abstract]
  • Product Specific References

  • *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  • *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  • *Beck Z, Balogh A, Kis A, Izsépi E, Cervenak L, László G, Bíró A, Liliom K, Mocsár G, Vámosi G, Füst G, Matko J: New cholesterol-specific antibodies remodel HIV-1 target cells' surface and inhibit their in vitro virus production. J Lipid Res. 2010 Feb;51(2):286-96. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

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  • Example Data


    MEM-75
    Fig. 1.

    Fig. 1. Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75) biotin; detection by Streptavidin-PE (cat. no. SAVP-C500).
    Total viable cells were used for analysis.













    MEM-75
    Fig. 2.

    Fig. 2. Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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