System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD59 / Protectin

MEM-43/5 (IgG2b)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-234-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-234-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.

Specificity

The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues.
The MEM-43/5 does not compete with most other CD59 antibodies.
HLDA V; WS Code AS S012

Regulatory Status

Immunogen

Thymocytes and T lymphocytes

Species Reactivity:

  • Human
  • Mouse

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution: 1-2 μg/ml
    Positive control: mouse spleen
    Application note: Non-reducing conditions.
  • Flow Cytometry
    Recommended dilution:1 μg/ml
    Positive control: blood
  • Immunoprecipitation
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 5 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ: Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers. Immunology. 1990 Sep;71(1):1-9. [Abstract] [Full Text]
  • *Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan BP: The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin Exp Immunol. 1991 Feb;83(2):251-6. [Abstract] [Full Text]
  • *Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE: CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15;153(6):2444-56. [Abstract]
  • *Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. J Immunol. 2004 Sep 15;173(6):3684-92. [Abstract] [Full Text]
  • Product Specific References

  • *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  • *Bodian DL, Davis SJ, Morgan BP, Rushmere NK.: Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59. J Exp Med. 1997 Feb 3;185(3):507-16. [Abstract] [Full Text]
  • *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. [Abstract] [Full Text]
  • *Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272(31):19242-7. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2b Isotype Control
  • Example Data


    mem-43/5
    Fig. 1. Western Blotting



    Fig. 1. Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5).
    Lane 1: original cell lysate
    Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43; cat. no. 11-233-C100)


    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy