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Mouse Monoclonal to CD58 / LFA-3

MEM-63 (IgG1)

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Background

CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.

Specificity

The antibody MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.
HLDA VI; WS Code AS A047

Regulatory Status

Immunogen

NALM-6 human pre-B cell line

Species Reactivity:

  • Human
  • Porcine

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:2 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Bayas MV, Schulten K, Leckband D: Forced detachment of the CD2-CD58 complex. Biophys J. 2003 Apr;84(4):2223-33. [Abstract] [Full Text]
  • *Veltroni M, De Zen L, Sanzari MC, Maglia O, Dworzak MN, Ratei R, Biondi A, Basso G, Gaipa G; I-BFM-ALL-FCM-MRD-Study Group: Expression of CD58 in normal, regenerating and leukemic bone marrow B cells: implications for the detection of minimal residual disease in acute lymphocytic leukemia. Haematologica. 2003 Nov;88(11):1245-52. [Abstract]
  • *Ariel O, Kukulansky T, Raz N, Hollander N: Distinct membrane localization and kinase association of the two isoforms of CD58. Cell Signal. 2004 Jun;16(6):667-73. [Abstract]
  • *Sheng L, Li J, Qi BT, Ji YQ, Meng ZJ, Xie M: Investigation on correlation between expression of CD58 molecule and severity of hepatitis B. World J Gastroenterol. 2006 Jul 14;12(26):4237-40. [Abstract]
  • Product Specific References

  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    mem-63
    Fig. 1. Flow Cytometry analysis



    Fig. 1. Surface staining of human peripheral blood cells with anti-CD58 (MEM-63) FITC.

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