categories: CD and Related Antigens (Human), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to CD56 / NCAM

MEM-188 (IgG2a)

Product No.	Form	Quantity	Price
11-231-C025	purified	0.025 mg	choose region
11-231-C100	purified	0.1 mg	choose region
1B-231-C025	biotin	0.025 mg	choose region
1B-231-C100	biotin	0.1 mg	choose region
1F-231-T025	FITC	25 tests	choose region
1F-231-T100	FITC	100 tests	choose region
1P-231-T025	PE	25 tests	choose region
1P-231-T100	PE	100 tests	choose region
1A-231-T025	APC	25 tests	choose region
1A-231-T100	APC	100 tests	choose region
T8-231-T025	PE-Cy™5	25 tests	choose region
T8-231-T100	PE-Cy™5	100 tests	choose region
PC-231-T025	PerCP	25 tests	choose region
PC-231-T100	PerCP	100 tests	choose region
T7-231-T025	PE-Cy™7	25 tests	choose region
T7-231-T100	PE-Cy™7	100 tests	choose region

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Background

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.

Entrez Gene: 11q23.1

Specificity

The antibody MEM-188 reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
HLDA VI; WS code A055
HLDA VI; WS Code NK26
HLDA VII; WS code 70077

Regulatory Status

RUO

Immunogen

KG-1 human acute myelogenous leukemia cell line

Species Reactivity: Human Non-Human Primates Negative Species:	Applications: Flow Cytometry Recommended dilution:3 μg/ml Immunoprecipitation Immunohistochemistry (paraffin sections) Recommended dilution: 1:50 Pretreatment: Heat retrieval (microwave oven) in citrate buffer, standard ABC visualization. Positive tissue: Positive membrane-specific reaction in adjacent peripheral nerve Staining of paraprostatic autonomic ganglion. Staining technique: Standard ABC technique (DAB+). Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH: Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991 Jun 15;146(12):4421-6. [Abstract] Jakovcevski I, Mo Z, Zecevic N: Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain. Neuroscience. 2007 Oct 26;149(2):328-37. [Abstract] [Full Text] Ohishi Y, Kaku T, Oya M, Kobayashi H, Wake N, Tsuneyoshi M: CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls. Gynecol Oncol. 2007 Oct;107(1):30-8. [Abstract] McCluggage WG, McKenna M, McBride HA: CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors. Int J Gynecol Pathol. 2007 Jul;26(3):322-7. [Abstract] Product Specific References Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002). Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74. [Abstract] [Full Text] Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62. [Abstract] [Full Text] Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. [Abstract] [Full Text] Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196(12):1617-26. [Abstract] [Full Text] Olsen I, Tollefsen S, Aagaard C, Reitan LJ, Bannantine JP, Andersen P, Sollid LM, Lundin KE: Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients. [Abstract] [Full Text] Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99. [Abstract] [Full Text] Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 5;12:2. [Abstract] [Full Text] Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61. [Abstract]

Species Reactivity:

Human
Non-Human Primates

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:3 μg/ml
Immunoprecipitation
Immunohistochemistry (paraffin sections)
Recommended dilution: 1:50
Pretreatment: Heat retrieval (microwave oven) in citrate buffer, standard ABC visualization.
Positive tissue: Positive membrane-specific reaction in adjacent peripheral nerve
Staining of paraprostatic autonomic ganglion.
Staining technique: Standard ABC technique (DAB+).

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH: Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991 Jun 15;146(12):4421-6. [Abstract]

*Jakovcevski I, Mo Z, Zecevic N: Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain. Neuroscience. 2007 Oct 26;149(2):328-37. [Abstract] [Full Text]

*Ohishi Y, Kaku T, Oya M, Kobayashi H, Wake N, Tsuneyoshi M: CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls. Gynecol Oncol. 2007 Oct;107(1):30-8. [Abstract]

*McCluggage WG, McKenna M, McBride HA: CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors. Int J Gynecol Pathol. 2007 Jul;26(3):322-7. [Abstract]

Product Specific References

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).

*Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74. [Abstract] [Full Text]

*Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62. [Abstract] [Full Text]

*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. [Abstract] [Full Text]

*Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196(12):1617-26. [Abstract] [Full Text]

*Olsen I, Tollefsen S, Aagaard C, Reitan LJ, Bannantine JP, Andersen P, Sollid LM, Lundin KE: Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients. [Abstract] [Full Text]

*Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99. [Abstract] [Full Text]

*Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 5;12:2. [Abstract] [Full Text]

*Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61. [Abstract]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG2a Isotype Control

Example Data

Fig. 1. Surface staining of human peripheral blood cells with anti-CD56 (MEM-188) FITC.

Fig. 2. Surface staining of human peripheral blood lymphocytes with anti-CD56 (MEM-188) PE.

Fig. 3. Surface staining of human peripheral blood lymphocytes with anti-CD56 (MEM-188) APC.

Fig. 4. Surface staining of human peripheral blood lymphocytes with anti-CD56 (MEM-188) PE-Cy^TM5.

Fig. 5. Surface staining of human peripheral blood lymphocytes with anti-CD56 (MEM-188) purified; GAM-APC.

Fig. 6. Surface staining of human peripheral blood lymphocytes with anti-CD56 (MEM-188) biotin; streptavidin-APC.

Fig. 7. Surface staining of human peripheral blood lymphocytes with anti-CD56 (MEM-188) PerCP.

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