categories: CD and Related Antigens (Human), CD and Related Antigens & MHC (Rodent), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to CD54 / ICAM-1

MEM-111 (IgG2a)

Product No.	Form	Quantity	Price
11-228-C025	purified	0.025 mg	choose region
11-228-C100	purified	0.1 mg	choose region
1P-228-T025	PE	25 tests	choose region
1P-228-T100	PE	100 tests	choose region

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- Manufacture on order

Background

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Entrez Gene (human): 19p13.3-p13.2

Specificity

The antibody MEM-111 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
HLDA VI; WS Code AS A049

Immunogen

Raji human Burkitt's lymphoma cell line

Species Reactivity: Human Rat Bovine Negative Species:	Applications: Flow Cytometry Recommended dilution: 2 μg/ml Western Blotting Positive control: RAJI cell line K562 leukemia cell line JY cell line Activated T-lymphocytes Application note: Non-reducing conditions. Immunohistochemistry (paraffin sections) Recommended dilution: 10 μg/ml Positive tissue: thymus, RE cells ELISA Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Leeuwenberg J.F.M. et al.: E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77, 543 (1992). [Abstract] Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Nagaraju K. et al.: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin Exp Immunol 113, 407 (1998). [Abstract] [Full Text] Tachimoto H. et al., Eotaxin-2 Alters Eosinophil Integrin Function via Mitogen-Activated Protein Kinases. Am J Respir Cell Mol Biol 26, 645 (2002). [Abstract] [Full Text] Burdick MM et al.: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am J Physiol Cell Physiol 284, C977 (2003). [Abstract] [Full Text] Hertel L. et al.: Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J Virol. 77, 7563 (2003). [Abstract] [Full Text] Bacáková L, Mares V, Lisá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9. [Abstract] Bacáková L, Mares V, Bottone MG, Pellicciari C, Lisá V, Svorcík V: Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture. J Biomed Mater Res. 2000 Mar 5;49(3):369-79. [Abstract] *Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179(12):8128-36. [Abstract] [Full Text]

Species Reactivity:

Human
Rat
Bovine

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 2 μg/ml
Western Blotting
Positive control:
RAJI cell line
K562 leukemia cell line
JY cell line
Activated T-lymphocytes
Application note: Non-reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: thymus, RE cells
ELISA

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Leeuwenberg J.F.M. et al.: E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77, 543 (1992). [Abstract]

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*Nagaraju K. et al.: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin Exp Immunol 113, 407 (1998). [Abstract] [Full Text]

*Tachimoto H. et al., Eotaxin-2 Alters Eosinophil Integrin Function via Mitogen-Activated Protein Kinases. Am J Respir Cell Mol Biol 26, 645 (2002). [Abstract] [Full Text]

*Burdick MM et al.: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am J Physiol Cell Physiol 284, C977 (2003). [Abstract] [Full Text]

*Hertel L. et al.: Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J Virol. 77, 7563 (2003). [Abstract] [Full Text]

*Bacáková L, Mares V, Lisá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9. [Abstract]

*Bacáková L, Mares V, Bottone MG, Pellicciari C, Lisá V, Svorcík V: Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture. J Biomed Mater Res. 2000 Mar 5;49(3):369-79. [Abstract]

*Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179(12):8128-36. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG2a Isotype Control

Example Data

Fig. 1.

Fig. 1. Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111. Lower bands represent tubulin as a loading control.

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