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Mouse Monoclonal to CD47 / Integrin Associated Protein

MEM-122 (IgM)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-225-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-225-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PB-225-T025 Pacific Blue™ 25 tests yes choose region PDF datasheetHTML datasheet buy
  PB-225-T100 Pacific Blue™ 100 tests yes choose region PDF datasheetHTML datasheet buy
  1F-225-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-225-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

Specificity

The antibody MEM-122 reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
HLDA VI; WS Code AS A051

Regulatory Status

Immunogen

COS-7 (African green monkey) cells

Species Reactivity:

  • Human
  • Non-Human Primates
  • Porcine

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:2 μg/ml
  • Western Blotting
    Application note: Non-reducing conditions.
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Barazi HO, Li Z, Cashel JA, Krutzsch HC, Annis DS, Mosher DF, Roberts DD: Regulation of integrin function by CD47 ligands. Differential effects on alpha vbeta 3 and alpha 4beta1 integrin-mediated adhesion. J Biol Chem. 2002 Nov 8;277(45):42859-66. [Abstract] [Full Text]
  • *Murata T, Ohnishi H, Okazawa H, Murata Y, Kusakari S, Hayashi Y, Miyashita M, Itoh H, Oldenborg PA, Furuya N, Matozaki T: CD47 promotes neuronal development through Src- and FRG/Vav2-mediated activation of Rac and Cdc42. J Neurosci. 2006 Nov 29;26(48):12397-407. [Abstract] [Full Text]
  • *Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages. Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5062-6. [Abstract] [Full Text]
  • *Orazizadeh M, Lee HS, Groenendijk BC, Millward-Sadler J, Wright MO, Lindberg FP, Salter DM. CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model. Arthritis Res Ther. 2008 Jan 10;10(1):R4 [Abstract]
  • *Isenberg JS, Pappan LK, Romeo MJ, Abu-Asab M, Tsokos M, Wink DA, Frazier WA, Roberts DD: Blockade of thrombospondin-1-CD47 interactions prevents necrosis of full thickness skin grafts. Ann Surg. 2008 Jan;247(1):180-90. [Abstract]
  • Product Specific References

  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *Smith RE, Patel V, Seatter SD, Deehan MR, Brown MH, Brooke GP, Goodridge HS, Howard CJ, Rigley KP, Harnett W, Harnett MM: A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes. Blood. 2003 Oct 1;102(7):2532-40. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgM Isotype Control
  • Example Data


    MEM-122



    Fig. 1. Surface staining of lysed and washed porcine peripheral blood with purified anti-CD47 (MEM-122) (detection by anti-mouse IgM FITC).
    Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; cat. no. 11-803-C100)
    Panel B, C, D - porcine PBMC sample stained with anti-CD47 (MEM-122)
    Cells in the granulocyte gate were used for analysis.


    MEM-122 PB


    Fig. 2. Surface staining of human peripheral blood with anti-CD47 (MEM-122) Pacific BlueTM.

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