System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD46 / Membrane Cofactor Protein

MEM-258 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-342-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-342-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  10-342-C025 azide free 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  10-342-C100 azide free 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-342-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-342-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PB-342-T025 Pacific Blue™ 25 tests yes choose region PDF datasheetHTML datasheet buy
  PB-342-T100 Pacific Blue™ 100 tests yes choose region PDF datasheetHTML datasheet buy
  1F-342-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-342-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-342-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-342-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-342-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-342-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  A7-342-T025 Alexa Fluor® 700 25 tests yes choose region PDF datasheetHTML datasheet buy
  A7-342-T100 Alexa Fluor® 700 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.

Specificity

The antibody MEM-258 recognizes an epitope on SCR4 (the membrane-proximal SCR) domain of CD46 (Membrane cofactor protein). CD46 is 56-66 kDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast; it is negative on erythrocytes.

Regulatory Status

Immunogen

HPB-ALL human T cell line

Species Reactivity:

  • Human
  • Bovine

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1 μg/ml
  • Immunoprecipitation
  • Western Blotting
    Application note: Non-reducing conditions.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Okada N, Liszewski MK, Atkinson JP, Caparon M: Membrane cofactor protein (CD46) is a keratinocyte receptor for the M protein of the group A streptococcus. Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2489-93. [Abstract] [Full Text]
  • *Marie JC, Astier AL, Rivailler P, Rabourdin-Combe C, Wild TF, Horvat B: Linking innate and acquired immunity: divergent role of CD46 cytoplasmic domains in T cell induced inflammation. Nat Immunol. 2002 Jul;3(7):659-66. [Abstract]
  • *Gaggar A, Shayakhmetov DM, Lieber A: CD46 is a cellular receptor for group B adenoviruses. Nat Med. 2003 Nov;9(11):1408-12. [Abstract]
  • *Liszewski MK, Kemper C, Price JD, Atkinson JP: Emerging roles and new functions of CD46. Springer Semin Immunopathol. 2005 Nov;27(3):345-58. [Abstract]
  • *Oliaro J, Pasam A, Waterhouse NJ, Browne KA, Ludford-Menting MJ, Trapani JA, Russell SM: Ligation of the cell surface receptor, CD46, alters T cell polarity and response to antigen presentation. Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18685-90. [Abstract] [Full Text]
  • Product Specific References

  • *Fleischli C, Verhaagh S, Havenga M, Sirena D, Schaffner W, Cattaneo R, Greber UF, Hemmi S: The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35. J Virol. 2005 Aug;79(15):10013-22. [Abstract] [Full Text]
  • *Weyand NJ, Lee SW, Higashi DL, Cawley D, Yoshihara P, So M: Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies. Infect Immun. 2006 Apr;74(4):2428-35. [Abstract] [Full Text]
  • *Fremeaux-Bacchi V, Moulton EA, Kavanagh D, Dragon-Durey MA, Blouin J, Caudy A, Arzouk N, Cleper R, Francois M, Guest G, Pourrat J, Seligman R, Fridman WH, Loirat C, Atkinson JP: Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome. J Am Soc Nephrol. 2006 Jul;17(7):2017-25. [Abstract] [Full Text]
  • *Loré K, Adams WC, Havenga MJ, Precopio ML, Holterman L, Goudsmit J, Koup RA: Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses. J Immunol. 2007 Aug 1;179(3):1721-9. [Abstract] [Full Text]
  • *Fleischli C, Sirena D, Lesage G, Havenga MJ, Cattaneo R, Greber UF, Hemmi S: Species B adenovirus serotypes 3, 7, 11 and 35 share similar binding sites on the membrane cofactor protein CD46 receptor. J Gen Virol. 2007 Nov;88(Pt 11):2925-34. [Abstract] [Full Text]
  • *Hoffmann D, Bayer W, Heim A, Potthoff A, Nettelbeck DM, Wildner O: Evaluation of twenty-one human adenovirus types and one infectivity-enhanced adenovirus for the treatment of malignant melanoma. J Invest Dermatol. 2008 Apr;128(4):988-98. [Abstract] [Full Text]
  • *Wang H, Tuve S, Erdman DD, Lieber A: Receptor usage of a newly emergent adenovirus type 14. Virology. 2009 May 10;387(2):436-41. [Abstract] [Full Text]
  • *Rebetz J, Na M, Su C, Holmqvist B, Edqvist A, Nyberg C, Widegren B, Salford LG, Sjögren HO, Arnberg N, Qian Q, Fan X: Fiber mediated receptor masking in non-infected bystander cells restricts adenovirus cell killing effect but promotes adenovirus host co-existence. PLoS One. 2009 Dec 29;4(12):e8484. [Abstract] [Full Text]
  • *Kälin S, Amstutz B, Gastaldelli M, Wolfrum N, Boucke K, Havenga M, DiGennaro F, Liska N, Hemmi S, Greber UF: Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35. J Virol. 2010 May;84(10):5336-50. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MEM-258



    Fig. 1. Surface staining of human peripheral blood cells with anti-CD46 (MEM-258) PE.



    MEM-258 Pacific Blue



    Fig. 2. Surface staining of human peripheral blood cells with anti-CD46 (MEM-258) Pacific BlueTM.


    MEM-258 biotin



    Fig. 3. Surface staining of human peripheral blood cells with anti-CD46 (MEM-258) biotin.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy