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Mouse Monoclonal to CD45RA

MEM-56 (IgG2b)

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Background

CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naïve T cells and normal plasma cells.

Specificity

The antibody MEM-56 reacts with CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.
HLDA IV; WS Code NL 907

Regulatory Status

Immunogen

Human thymocytes and T lymphocytes.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1-4 μg/ml
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    No pre-treatment of tissue sections is essential.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302. [Abstract] [Full Text]
  • *Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65. [Abstract] [Full Text]
  • *Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176(6):3417-25. [Abstract] [Full Text]
  • *Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90. [Abstract]
  • *Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19 [Abstract]
  • Product Specific References

  • *Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 1989;35(5):289-97. [Abstract]
  • *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2b Isotype Control
  • Example Data


    mem-56


    Fig. 1. Western Blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line.
    Lane 1: immunostaining with anti-human CD45 control antibody
    Lane 2: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under non-reducing conditions
    Lane 3: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under reducing conditions


    MEM-56 purified MC

    Fig. 2. Surface staining (mass cytometry) of human peripheral blood with anti-CD45RA (MEM-56) 163Dy. Gated on singlets.

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