- Canine (Dog)
- Flow Cytometry
Recommended dilution: 4 μg/ml
- Western Blotting
Recommended dilution: 2 μg/ml, 60 min on vertical incubator
Kg-1a human acute leukemia cell lysate
JURKAT human leukemia T-cell lysate
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).
- Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: uterus, myometrium
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A: Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging. J Biol Chem. 2007 Dec 12 [Abstract] [Full Text]
*Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA. CD44 regulates macrophage recruitment to the lung in lipopolysaccharide-induced airway disease. Am J Respir Cell Mol Biol. 2007 Aug;37(2):248-53.
*Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G. Down-regulation of CD44 contributes to the differentiation of HL-60 cells induced by ATRA or HMBA. Cell Mol Immunol. 2007 Feb;4(1):59-63.
*Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp Mol Pathol. 2007 Dec;83(3):341-6.
*Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT
phosphorylation. Exp Mol Pathol. 2007 Oct;83(2):207-15.
Product Specific References
*Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987).