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Mouse Monoclonal to CD44 / Pgp-1

MEM-263 (IgG1)

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  11-341-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1F-341-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-341-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-341-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-341-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-341-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Specificity

The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
HLDA III; WS Code T 155

Regulatory Status

Immunogen

COS-7 cells (African Green Monkey).

Species Reactivity:

  • Human
  • Porcine
  • Canine (Dog)

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 4 μg/ml
  • Immunoprecipitation
  • Western Blotting
    Recommended dilution: 2 μg/ml, 60 min on vertical incubator
    Positive control:
    Kg-1a human acute leukemia cell lysate
    JURKAT human leukemia T-cell lysate
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
    Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Positive tissue: uterus, myometrium
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A: Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging. J Biol Chem. 2007 Dec 12 [Abstract] [Full Text]
  • *Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA. CD44 regulates macrophage recruitment to the lung in lipopolysaccharide-induced airway disease. Am J Respir Cell Mol Biol. 2007 Aug;37(2):248-53. [Abstract]
  • *Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G. Down-regulation of CD44 contributes to the differentiation of HL-60 cells induced by ATRA or HMBA. Cell Mol Immunol. 2007 Feb;4(1):59-63. [Abstract]
  • *Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp Mol Pathol. 2007 Dec;83(3):341-6. [Abstract]
  • *Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation. Exp Mol Pathol. 2007 Oct;83(2):207-15. [Abstract]
  • Product Specific References

  • *Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987).
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MEM-263
    Fig. 1. Western Blotting



    Fig. 1.
    Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263).
    Lane 1: lysate of human PBL
    Lane 2: lysate of canine PBL
    Lane 3: lysate of porcine PBL

    MEM-263 FC



    Fig. 2.
    Flow cytometry analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (MEM-263) PE conjugate.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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