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Mouse Monoclonal to CD38

HIT2 (IgG1)

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Background

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.

Specificity

The antibody HIT2 reacts with CD38 (T10), a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells.
HLDA III; WS Code T 155

Regulatory Status

Immunogen

Human thymocytes in foetus

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Western Blotting
    Recommended dilution: 2 μg/ml
    Positive control: RAJI human cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Boil for 5 min.
    Application note: Non-reducing conditions.
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Cakir-Kiefer C, Muller-Steffner H, Oppenheimer N, Schuber F: Kinetic competence of the cADP-ribose-CD38 complex as an intermediate in the CD38/NAD+ glycohydrolase-catalysed reactions: implication for CD38 signalling. Biochem J. 2001 Sep 1;358(Pt 2):399-406. [Abstract] [Full Text]
  • *Lund FE, Muller-Steffner H, Romero-Ramirez H, Moreno-García ME, Partida-Sánchez S, Makris M, Oppenheimer NJ, Santos-Argumedo L, Schuber F: CD38 induces apoptosis of a murine pro-B leukemic cell line by a tyrosine kinase-dependent but ADP-ribosyl cyclase- and NAD glycohydrolase-independent mechanism. Int Immunol. 2006 Jul;18(7):1029-42. [Abstract]
  • *Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE. Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction. J Immunol. 2007 Dec 1;179(11):7827-39. [Abstract]
  • Product Specific References

  • *Leukocyte Typing III., McMichael AJ et al (Eds.), Oxford University Press (1987).
  • *Rozková D, Novotná L, Pytlík R, Hochová I, Kozák T, Bartůnková J, Spísek R: Toll-like receptors on B-CLL cells: expression and functional consequences of their stimulation. Int J Cancer. 2010 Mar 1;126(5):1132-43. [Abstract]
  • *Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15;109(6):2545-52. [Abstract] [Full Text]
  • Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr;15(4):e61-71. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    hit2


    Fig. 1. Surface staining of PHA stimulated human peripheral blood lymphocytes with anti-human CD38 (HIT2) PE.


    HIT2 AF700 FC


    Fig. 2. Surface staining of human peripheral blood with anti-human CD38 (HIT2) Alexa Fluor® 700.


    HIT2 FITC


    Fig. 3. Surface staining of human peripheral blood with anti-human CD38 (HIT2) FITC.


    HIT2 PE-Cy7


    Fig. 4. Surface staining of human peripheral blood with anti-human CD38 (HIT2) PE-CyTM7.


    HIT2 PE-Cy5


    Fig. 5. Surface staining of human peripheral blood with anti-human CD38 (HIT2) PE-CyTM5.


    HIT2 biotin


    Fig. 6. Surface staining of human peripheral blood with anti-human CD38 (HIT2) biotin / streptavidin-APC.


    HIT2 PerCP


    Fig. 7. Surface staining of human peripheral blood with anti-human CD38 (HIT2) PerCP.

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