categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD34 / Mucosialin

4H11[APG] (IgG1)

Product No.	Form	Quantity	Price
11-297-C025	purified	0.025 mg	choose region
11-297-C100	purified	0.1 mg	choose region
1B-297-C025	biotin	0.025 mg	choose region
1B-297-C100	biotin	0.1 mg	choose region
PB-297-T025	Pacific Blue™	25 tests	choose region
PB-297-T100	Pacific Blue™	100 tests	choose region
1F-297-T025	FITC	25 tests	choose region
1F-297-T100	FITC	100 tests	choose region
A4-297-T025	Alexa Fluor® 488	25 tests	choose region
A4-297-T100	Alexa Fluor® 488	100 tests	choose region
1P-297-T025	PE	25 tests	choose region
1P-297-T100	PE	100 tests	choose region
1A-297-T025	APC	25 tests	choose region
1A-297-T100	APC	100 tests	choose region
A7-297-T025	Alexa Fluor® 700	25 tests	choose region
A7-297-T100	Alexa Fluor® 700	100 tests	choose region
T7-297-T025	PE-Cy™7	25 tests	choose region
T7-297-T100	PE-Cy™7	100 tests	choose region

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Background

CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Entrez Gene: 1q32

Specificity

The antibody 4H11[APG] reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
HLDA VI; WS Code M MA58

Regulatory Status

RUO

Immunogen

Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution: 2 μg/ml Western Blotting Recommended dilution: 2 μg/ml Positive control: Kg-1a human leukemia cell lysate Negative control: JURKAT human leukemia T-cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. Immunohistochemistry (paraffin sections) Recommended dilution: 10 μg/ml Positive tissue: placenta endothelium Immunocytochemistry Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1. J Hematother Stem Cell Res. 2001 Dec;10(6):863-71. [Abstract] Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan 1;101(1):112-8. [Abstract] [Full Text] Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L. Stem Cells Dev. 2005 Oct;14(5):470-7. [Abstract] Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun;15(3):305-13. [Abstract] Product Specific References Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Elknerová K, Lacinová Z, Soucek J, Marinov I, Stöckbauer P: Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines. Neoplasma. 2007;54(4):311-20. [Abstract]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 2 μg/ml
Western Blotting
Recommended dilution: 2 μg/ml
Positive control: Kg-1a human leukemia cell lysate
Negative control: JURKAT human leukemia T-cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: Non-reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: placenta endothelium
Immunocytochemistry

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1. J Hematother Stem Cell Res. 2001 Dec;10(6):863-71. [Abstract]

*Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan 1;101(1):112-8. [Abstract] [Full Text]

*Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L. Stem Cells Dev. 2005 Oct;14(5):470-7. [Abstract]

*Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun;15(3):305-13. [Abstract]

Product Specific References

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*Elknerová K, Lacinová Z, Soucek J, Marinov I, Stöckbauer P: Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines. Neoplasma. 2007;54(4):311-20. [Abstract]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG1 Isotype Control

Example Data

Fig. 1. Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. Total viable cells were used for analysis.

Fig. 2. Detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE.

Fig. 3. Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Alexa Fluor^® 700.

Fig. 4. Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Pacific Blue^TM.

Fig. 5. Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) PE-Cy^TM7.

Fig. 6. Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Alexa Fluor^® 488.

Fig. 7. Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) APC.

Fig. 8. Surface staining of CD34+ cells in human peripheral blood with anti-CD34 (4H11[APG]) biotin / streptavidin-APC.

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