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Mouse Monoclonal to CD34 / Mucosialin

4H11[APG] (IgG1)

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Background

CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Specificity

The antibody 4H11[APG] reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
HLDA VI; WS Code M MA58

Regulatory Status

Immunogen

Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 2 μg/ml
  • Western Blotting
    Recommended dilution: 2 μg/ml
    Positive control: Kg-1a human leukemia cell lysate
    Negative control: JURKAT human leukemia T-cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
    Application note: Non-reducing conditions.
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Positive tissue: placenta endothelium
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1. J Hematother Stem Cell Res. 2001 Dec;10(6):863-71. [Abstract]
  • *Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan 1;101(1):112-8. [Abstract] [Full Text]
  • *Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L. Stem Cells Dev. 2005 Oct;14(5):470-7. [Abstract]
  • *Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun;15(3):305-13. [Abstract]
  • Product Specific References

  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *Elknerová K, Lacinová Z, Soucek J, Marinov I, Stöckbauer P: Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines. Neoplasma. 2007;54(4):311-20. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    4h11


    Fig. 1. Surface staining of Kg-1a human acute myelogenous leukemia cell line with anti-human CD34 (4H11[APG]) FITC. Total viable cells were used for analysis.


    4H11 ICC


    Fig. 2. Detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human CD34 (4H11[APG]) PE. 



    4h11 AF700


    Fig. 3. Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Alexa Fluor® 700. 



    4H11 Pacific Blue


    Fig. 4. Surface staining of CD34+ cells in human peripheral blood with anti-human CD34 (4H11[APG]) Pacific BlueTM

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