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Mouse Monoclonal to CD31 / PECAM-1

MEM-05 (IgG1)

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Background

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

Specificity

The antibody MEM-05 reacts with CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.

Regulatory Status

Immunogen

Leukocytes of patient suffering from LGL-type leukaemia

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1-10 μg/ml
  • Western Blotting
    Application note: Non-reducing conditions
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Newman DK, Hamilton C, Newman PJ: Inhibition of antigen-receptor signaling by Platelet Endothelial Cell Adhesion Molecule-1 (CD31) requires functional ITIMs, SHP-2, and p56(lck). Blood. 2001 Apr 15;97(8):2351-7. [Abstract] [Full Text]
  • *Wilkinson R, Lyons AB, Roberts D, Wong MX, Bartley PA, Jackson DE: Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) acts as a regulator of B-cell development, B-cell antigen receptor (BCR)-mediated activation, and autoimmune disease. Blood. 2002 Jul 1;100(1):184-93. [Abstract] [Full Text]
  • *Jackson DE: The unfolding tale of PECAM-1. FEBS Lett. 2003 Apr 10;540(1-3):7-14. [Abstract]
  • *Wee JL, Jackson DE: The Ig-ITIM superfamily member PECAM-1 regulates the "outside-in" signaling properties of integrin alpha(IIb)beta3 in platelets. Blood. 2005 Dec 1;106(12):3816-23. [Abstract] [Full Text]
  • *Woodfin A, Voisin MB, Nourshargh S: PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler Thromb Vasc Biol. 2007 Dec;27(12):2514-23. [Abstract]
  • *Wong MX, Hayball JD, Jackson DE: PECAM-1-regulated signalling thresholds control tolerance in anergic transgenic B-cells. Mol Immunol. 2007 Oct 29 [Abstract]
  • Product Specific References

  • *Prager E, Staffler G, Majdic O, Saemann M, Godar S, Zlabinger G, Stockinger H: Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells. J Immunol. 2001 Feb 15;166(4):2364-71. [Abstract] [Full Text]
  • *Cârţână T, Săftoiu A, Gruionu LG, Gheonea DI, Pirici D, Georgescu CV, Ciocâlteu A, Gruionu G: Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti-CD31 antibodies. PLoS One. 2012;7(12):e52815. doi: 10.1371/journal.pone.0052815. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MEM-05
    Fig. 1. Flow Cytometry analysis


    Fig. 1. Surface staining of lysed and wash human peripheral blood with purified anti-human CD31 (MEM-05) (detection by Goat anti-mouse IgG1 PE).

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