System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD29 / Integrin beta1 chain

MEM-101A (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-219-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-219-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-219-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-219-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PB-219-T025 Pacific Blue™ 25 tests yes choose region PDF datasheetHTML datasheet buy
  PB-219-T100 Pacific Blue™ 100 tests yes choose region PDF datasheetHTML datasheet buy
  1F-219-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-219-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-219-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-219-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-219-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-219-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.

Specificity

The antibody MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells).
HLDA VI; WS Code AS A048

Regulatory Status

Immunogen

Raji Burkitt's lymphoma cell line

Species Reactivity:

  • Human
  • Porcine
  • Canine (Dog)

Negative Species:

  • Mouse

Applications:

  • Flow Cytometry
    Recommended dilution:2 μg/ml
  • Immunoprecipitation
  • Western Blotting
    Recommended dilution:2 μg/ml
    Positive control:JURKAT human leukemia T-cell lysate
    Kg-1a human leukemia cell lysate
    Sample preparation:buffer with laurylmaltoside, 2 x non-reducing SDS
    Application note:Non-reducing conditions. SDS-PAGE (6% separating gel, 4% stacking gel). Recommended secondary antibody - anti mouse IgG/HRP, dilution 1:2000, 60 min on vertical incubator.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Wencel-Drake JD, Dieter MG, Lam SC: Immunolocalization of beta 1 integrins in platelets and platelet-derived microvesicles. Blood. 1993 Aug 15;82(4):1197-203. [Abstract] [Full Text]
  • *Werr J, Johansson J, Eriksson EE, Hedqvist P, Ruoslahti E, Lindbom L: Integrin alpha(2)beta(1) (VLA-2) is a principal receptor used by neutrophils for locomotion in extravascular tissue. Blood. 2000 Mar 1;95(5):1804-9. [Abstract] [Full Text]
  • *Peng Q, Lai D, Nguyen TT, Chan V, Matsuda T, Hirst SJ: Multiple beta 1 integrins mediate enhancement of human airway smooth muscle cytokine secretion by fibronectin and type I collagen. J Immunol. 2005 Feb 15;174(4):2258-64. [Abstract] [Full Text]
  • *Lundberg S, Lindholm J, Lindbom L, Hellström PM, Werr J: Integrin alpha2beta1 regulates neutrophil recruitment and inflammatory activity in experimental colitis in mice. Inflamm Bowel Dis. 2006 Mar;12(3):172-7. [Abstract] [Full Text]
  • *Morozevich GE, Kozlova NI, Preobrazhenskaya ME, Ushakova NA, Eltsov IA, Shtil AA, Berman AE: The role of beta1 integrin subfamily in anchorage-dependent apoptosis of breast carcinoma cells differing in multidrug resistance. Biochemistry (Mosc). 2006 May;71(5):489-95. [Abstract] [Full Text]
  • Product Specific References

  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *Plánka L, Necas A, Srnec R, Rauser P, Starý D, Jancár J, Amler E, Filová E, Hlucilová J, Kren L, Gál P: Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs. Physiol Res. 2009;58(6):885-93. [Abstract]
  • *Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MEM-101A
    Fig. 1. Western Blotting



    Fig. 1.
    Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD29 (MEM-101A).
    Lane 1: lysate of human PBL
    Lane 2: lysate of canine PBL
    Lane 3: lysate of porcine PBL

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy