System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD21

LT21 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-306-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-306-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PB-306-T025 Pacific Blue™ 25 tests yes choose region PDF datasheetHTML datasheet buy
  PB-306-T100 Pacific Blue™ 100 tests yes choose region PDF datasheetHTML datasheet buy
  1F-306-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-306-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-306-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-306-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  T8-306-T025 PE-Cy™5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T8-306-T100 PE-Cy™5 100 tests yes choose region PDF datasheetHTML datasheet buy
  A6-306-T025 Alexa Fluor® 647 25 tests yes choose region PDF datasheetHTML datasheet buy
  A6-306-T100 Alexa Fluor® 647 100 tests yes choose region PDF datasheetHTML datasheet buy
  T7-306-T025 PE-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
  T7-306-T100 PE-Cy™7 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Specificity

The antibody LT21 reacts with CD21 (CR2), a 145 kDa transmembrane glycoprotein (complement C3d receptor - C3dR) expressed on B lymphocytes, follicular dendritic cells, some epithelial cells and a subsets of T lymphocytes. It is not expressed on immature B cells.
HLDA VI; WS Code B CD21.1

Regulatory Status

Immunogen

IM9 human B-lymphoblastoid cell line

Species Reactivity:

  • Human
  • Porcine
  • Bovine
  • Canine (Dog)

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:2 μg/ml
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Roozendaal R, Carroll MC: Complement receptors CD21 and CD35 in humoral immunity. Immunol Rev. 2007 Oct;219:157-66. [Abstract]
  • *Twohig J, Kulik L, Haluszczak C, Reuter J, Rossbach A, Bull M, Holers VM, Marchbank KJ: Defective B cell ontogeny and immune response in human complement receptor 2 (CR2, CD21) transgenic mice is partially recovered in the absence of C3. Mol Immunol. 2007 Jul;44(13):3434-44. [Abstract] [Full Text]
  • *Kasprzak A, Spachacz R, Wachowiak J, Stefanska K, Zabel M: Epstein-Barr virus (EBV) infection in B-cell non-Hodgkin's lymphomas in children: virus latency and its correlation with CD21 and CD23 molecules. Folia Histochem Cytobiol. 2007;45(3):169-79. [Abstract]
  • *Zabel MD, Heikenwalder M, Prinz M, Arrighi I, Schwarz P, Kranich J, von Teichman A, Haas KM, Zeller N, Tedder TF, Weis JH, Aguzzi A: Stromal complement receptor CD21/35 facilitates lymphoid prion colonization and pathogenesis. J Immunol. 2007 Nov 1;179(9):6144-52. [Abstract]
  • *Singh A, Blank M, Shoenfeld Y, Illges H: Antiphospholipid syndrome patients display reduced titers of soluble CD21 in their sera irrespective of circulating anti-beta2-glycoprotein-I autoantibodies. Rheumatol Int. 2008 Jan 3 [Abstract]
  • Product Specific References

  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):56-62. [Abstract]
  • *Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    LT21 FC

    Fig. 1. Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD21 (LT21) antibody.


    LT21 PE-Cy7

    Fig. 2. Surface staining of human peripheral blood leukocytes with anti-CD21 (LT21) PE-Cy7.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy