categories: CD and Related Antigens (Human), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to CD21

LT21 (IgG1)

Product No.	Form	Quantity	Price
11-306-C025	purified	0.025 mg	choose region
11-306-C100	purified	0.1 mg	choose region
PB-306-T025	Pacific Blue™	25 tests	choose region
PB-306-T100	Pacific Blue™	100 tests	choose region
1F-306-T025	FITC	25 tests	choose region
1F-306-T100	FITC	100 tests	choose region
1P-306-T025	PE	25 tests	choose region
1P-306-T100	PE	100 tests	choose region
A6-306-T025	Alexa Fluor® 647	25 tests	choose region
A6-306-T100	Alexa Fluor® 647	100 tests	choose region

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Background

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Entrez Gene (human): 1q32

Specificity

The antibody LT21 reacts with CD21 (CR2), a 145 kDa transmembrane glycoprotein (complement C3d receptor - C3dR) expressed on B lymphocytes, follicular dendritic cells, some epithelial cells and a subsets of T lymphocytes. It is not expressed on immature B cells.
HLDA VI; WS Code B CD21.1

Immunogen

IM9 human B-lymphoblastoid cell line

Species Reactivity: Human Porcine Bovine Canine (Dog) Negative Species:	Applications: Flow Cytometry Immunohistochemistry (frozen sections) Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Roozendaal R, Carroll MC: Complement receptors CD21 and CD35 in humoral immunity. Immunol Rev. 2007 Oct;219:157-66. [Abstract] Twohig J, Kulik L, Haluszczak C, Reuter J, Rossbach A, Bull M, Holers VM, Marchbank KJ: Defective B cell ontogeny and immune response in human complement receptor 2 (CR2, CD21) transgenic mice is partially recovered in the absence of C3. Mol Immunol. 2007 Jul;44(13):3434-44. [Abstract] [Full Text] Kasprzak A, Spachacz R, Wachowiak J, Stefanska K, Zabel M: Epstein-Barr virus (EBV) infection in B-cell non-Hodgkin's lymphomas in children: virus latency and its correlation with CD21 and CD23 molecules. Folia Histochem Cytobiol. 2007;45(3):169-79. [Abstract] Zabel MD, Heikenwalder M, Prinz M, Arrighi I, Schwarz P, Kranich J, von Teichman A, Haas KM, Zeller N, Tedder TF, Weis JH, Aguzzi A: Stromal complement receptor CD21/35 facilitates lymphoid prion colonization and pathogenesis. J Immunol. 2007 Nov 1;179(9):6144-52. [Abstract] Singh A, Blank M, Shoenfeld Y, Illges H: Antiphospholipid syndrome patients display reduced titers of soluble CD21 in their sera irrespective of circulating anti-beta2-glycoprotein-I autoantibodies. Rheumatol Int. 2008 Jan 3 [Abstract] Product Specific References Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). *Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):56-62. [Abstract]

Species Reactivity:

Human
Porcine
Bovine
Canine (Dog)

Negative Species:

Applications:

Flow Cytometry
Immunohistochemistry (frozen sections)

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Roozendaal R, Carroll MC: Complement receptors CD21 and CD35 in humoral immunity. Immunol Rev. 2007 Oct;219:157-66. [Abstract]

*Twohig J, Kulik L, Haluszczak C, Reuter J, Rossbach A, Bull M, Holers VM, Marchbank KJ: Defective B cell ontogeny and immune response in human complement receptor 2 (CR2, CD21) transgenic mice is partially recovered in the absence of C3. Mol Immunol. 2007 Jul;44(13):3434-44. [Abstract] [Full Text]

*Kasprzak A, Spachacz R, Wachowiak J, Stefanska K, Zabel M: Epstein-Barr virus (EBV) infection in B-cell non-Hodgkin's lymphomas in children: virus latency and its correlation with CD21 and CD23 molecules. Folia Histochem Cytobiol. 2007;45(3):169-79. [Abstract]

*Zabel MD, Heikenwalder M, Prinz M, Arrighi I, Schwarz P, Kranich J, von Teichman A, Haas KM, Zeller N, Tedder TF, Weis JH, Aguzzi A: Stromal complement receptor CD21/35 facilitates lymphoid prion colonization and pathogenesis. J Immunol. 2007 Nov 1;179(9):6144-52. [Abstract]

*Singh A, Blank M, Shoenfeld Y, Illges H: Antiphospholipid syndrome patients display reduced titers of soluble CD21 in their sera irrespective of circulating anti-beta2-glycoprotein-I autoantibodies. Rheumatol Int. 2008 Jan 3 [Abstract]

Product Specific References

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):56-62. [Abstract]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG1 Isotype Control

Example Data

Fig. 1.

Fig. 1. Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and anti-CD21 (LT21) antibody.

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