System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD19

LT19 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-305-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-305-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-305-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-305-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PB-305-T025 Pacific Blue™ 25 tests yes choose region PDF datasheetHTML datasheet buy
  PB-305-T100 Pacific Blue™ 100 tests yes choose region PDF datasheetHTML datasheet buy
  1F-305-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-305-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-305-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-305-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  T5-305-T025 PE-DyLight® 594 25 tests yes choose region PDF datasheetHTML datasheet buy
  T5-305-T100 PE-DyLight® 594 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-305-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-305-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  PC-305-T025 PerCP 25 tests yes choose region PDF datasheetHTML datasheet buy
  PC-305-T100 PerCP 100 tests yes choose region PDF datasheetHTML datasheet buy
  T7-305-T025 PE-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
  T7-305-T100 PE-Cy™7 100 tests yes choose region PDF datasheetHTML datasheet buy
  T4-305-T025 APC-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
  T4-305-T100 APC-Cy™7 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

Specificity

The antibody LT19 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
HLDA 10

Regulatory Status

Immunogen

Daudi human Burkitt lymphoma cell line

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:5 μg/ml
  • Immunoprecipitation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Fujimoto M, Poe JC, Jansen PJ, Sato S, Tedder TF: CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation. J Immunol. 1999 Jun 15;162(12):7088-94. [Abstract] [Full Text]
  • *Inabe K, Kurosaki T: Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15;99(2):584-9. [Abstract] [Full Text]
  • *van Zelm MC, Reisli I, van der Burg M, Castaño D, van Noesel CJ, van Tol MJ, Woellner C, Grimbacher B, Patiño PJ, van Dongen JJ, Franco JL: An antibody-deficiency syndrome due to mutations in the CD19 gene. N Engl J Med. 2006 May 4;354(18):1901-12. [Abstract] [Full Text]
  • *Shi X, Xie C, Chang S, Zhou XJ, Tedder T, Mohan C: CD19 hyperexpression augments Sle1-induced humoral autoimmunity but not clinical nephritis. Arthritis Rheum. 2007 Sep;56(9):3057-69. [Abstract]
  • Product Specific References

  • *Elias F, Flo J, Lopez RA, Zorzopulos J, Montaner A, Rodriguez JM: Strong cytosine-guanosine-independent immunostimulation in humans and other primates by synthetic oligodeoxynucleotides with PyNTTTTGT motifs. J Immunol. 2003 Oct 1;171(7):3697-704. [Abstract] [Full Text]
  • *Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74. Epub 2005 Jul 26. [Abstract] [Full Text]
  • *Stehlíková O, Chovancová J, Tichý B, Krejčí M, Brychtová Y, Panovská A, Francová Skuhrová H, Burčková K, Borský M, Loja T, Mayer J, Pospíšilová S, Doubek M: Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8-color flow cytometry protocol in routine hematological practice. Int J Lab Hematol. 2013 Sep 13. doi: 10.1111/ijlh.12149. [Abstract]
  • *Kayserova J, Vcelakova J, Stechova K, Dudkova E, Hromadkova H, Sumnik Z, Kolouskova S, Spisek R, Sediva A: Decreased dendritic cell numbers but increased TLR9-mediated interferon-alpha production in first degree relatives of type 1 diabetes patients. Clin Immunol. 2014 Jul;153(1):49-55. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    lt19


    Fig. 1. Surface staining of human peripheral blood cells with anti-human CD19 (LT19) APC.


    LT19 APC-Cy7


    Fig. 2. Surface staining of human peripheral blood cells with anti-human CD19 (LT19) APC-CyTM7. 


    LT19 biotin


    Fig. 3. Surface staining of human peripheral blood cells with anti-human CD19 (LT19) biotin. 

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy