categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD18 / Integrin beta2 subunit

MEM-48 (IgG1)

Product No.	Form	Quantity	Price
11-216-C025	purified	0.025 mg	choose region
11-216-C100	purified	0.1 mg	choose region
12-216-C100	low endotoxin	0.1 mg	choose region
1B-216-C025	biotin	0.025 mg	choose region
1B-216-C100	biotin	0.1 mg	choose region
1F-216-T025	FITC	25 tests	choose region
1F-216-T100	FITC	100 tests	choose region
1P-216-T025	PE	25 tests	choose region
1P-216-T100	PE	100 tests	choose region
1A-216-T025	APC	25 tests	choose region
1A-216-T100	APC	100 tests	choose region
A7-216-T025	Alexa Fluor® 700	25 tests	choose region
A7-216-T100	Alexa Fluor® 700	100 tests	choose region

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Background

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Entrez Gene: 21q22.3

Specificity

The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Immunogen

Leukocytes of a patient suffering from a LGL-type leukemia.

Species Reactivity: Human Negative Species: Canine (Dog)	Applications: Flow Cytometry Recommended dilution: 2 μg/ml Immunoprecipitation Western Blotting Recommended dilution: 2 μg/ml Positive control: Kg-1a human leukemia cell lysate Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). Immunohistochemistry (paraffin sections) Recommended dilution: 10 μg/ml Positive tissue: spleen, microglia Immunohistochemistry (frozen sections) Functional Application The antibody MEM-48 induces high-affinity conformation of LFA-1 complex. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Gao JX, Issekutz AC: Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. Immunology. 1996 Jul;88(3):463-70. [Abstract] [Full Text] Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V: Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun. 2000 Aug 28;275(2):295-9. [Abstract] Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol. 1998 Jun;28(6):1970-9. [Abstract] Kess D, Peters T, Zamek J, Wickenhauser C, Tawadros S, Loser K, Varga G, Grabbe S, Nischt R, Sunderkötter C, Müller W, Krieg T, Scharffetter-Kochanek K: CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J Immunol. 2003 Dec 1;171(11):5697-706. [Abstract] [Full Text] Solovjov DA, Pluskota E, Plow EF: Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2. J Biol Chem. 2005 Jan 14;280(2):1336-45. [Abstract] [Full Text] Peters T, Sindrilaru A, Wang H, Oreshkova T, Renkl AC, Kess D, Scharffetter-Kochanek K: CD18 in monogenic and polygenic inflammatory processes of the skin. J Investig Dermatol Symp Proc. 2006 Sep;11(1):7-15. [Abstract] Dassanayake RP, Maheswaran SK, Srikumaran S: Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects. Infect Immun. 2007 Oct;75(10):5004-10. [Abstract] Product Specific References Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha). 1990;36(1):41-50. [Abstract] Larson RS, Springer TA: Structure and function of leukocyte integrins. Immunol Rev. 1990 Apr;114:181-217. Stefanova I, Horejsi V: Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules. J Immunol. 1991 Sep 1;147(5):1587-92. [Abstract] Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95). J Immunol. 2000 Jun 1;164(11):5928-34. [Abstract] [Full Text] Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15;93(10):3505-11. [Abstract] [Full Text] Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT: Increased phagocyte Fc gammaRI expression and improved Fc gamma-receptor-mediated phagocytosis after in vivo recombinant human interferon-gamma treatment of normal human subjects. Blood. 1997 Oct 15;90(8):3187-94. [Abstract] [Full Text] Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113(2):358-69. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Canine (Dog)

Applications:

Flow Cytometry
Recommended dilution: 2 μg/ml
Immunoprecipitation
Western Blotting
Recommended dilution: 2 μg/ml
Positive control: Kg-1a human leukemia cell lysate
Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS
Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: spleen, microglia
Immunohistochemistry (frozen sections)
Functional Application
The antibody MEM-48 induces high-affinity conformation of LFA-1 complex.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Gao JX, Issekutz AC: Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. Immunology. 1996 Jul;88(3):463-70. [Abstract] [Full Text]

*Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V: Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun. 2000 Aug 28;275(2):295-9. [Abstract]

*Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol. 1998 Jun;28(6):1970-9. [Abstract]

*Kess D, Peters T, Zamek J, Wickenhauser C, Tawadros S, Loser K, Varga G, Grabbe S, Nischt R, Sunderkötter C, Müller W, Krieg T, Scharffetter-Kochanek K: CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J Immunol. 2003 Dec 1;171(11):5697-706. [Abstract] [Full Text]

*Solovjov DA, Pluskota E, Plow EF: Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2. J Biol Chem. 2005 Jan 14;280(2):1336-45. [Abstract] [Full Text]

*Peters T, Sindrilaru A, Wang H, Oreshkova T, Renkl AC, Kess D, Scharffetter-Kochanek K: CD18 in monogenic and polygenic inflammatory processes of the skin. J Investig Dermatol Symp Proc. 2006 Sep;11(1):7-15. [Abstract]

*Dassanayake RP, Maheswaran SK, Srikumaran S: Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects. Infect Immun. 2007 Oct;75(10):5004-10. [Abstract]

Product Specific References

*Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha). 1990;36(1):41-50. [Abstract]

*Larson RS, Springer TA: Structure and function of leukocyte integrins. Immunol Rev. 1990 Apr;114:181-217.

*Stefanova I, Horejsi V: Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules. J Immunol. 1991 Sep 1;147(5):1587-92. [Abstract]

*Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95). J Immunol. 2000 Jun 1;164(11):5928-34. [Abstract] [Full Text]

*Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15;93(10):3505-11. [Abstract] [Full Text]

*Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT: Increased phagocyte Fc gammaRI expression and improved Fc gamma-receptor-mediated phagocytosis after in vivo recombinant human interferon-gamma treatment of normal human subjects. Blood. 1997 Oct 15;90(8):3187-94. [Abstract] [Full Text]

*Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113(2):358-69. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG1 Isotype Control

Example Data

Fig. 1A Flow Cytometry analysis

Fig. 1 Surface staining of human peripheral blood cells with anti-human CD18 (MEM-48) PE. Cells in the granulocyte gate were used for analysis.

Fig. 1B Western Blotting

Fig. 2 Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 (MEM-48).
Lane 1: KG-1a human acute myeloid leukemia cell line
Lane 2: HPB-ALL human peripheral blood T cell leukemia cell line

Fig. 3.

Fig. 3 Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).
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