System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD18 / Integrin beta2 subunit

MEM-48 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-216-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-216-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  12-216-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-216-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-216-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-216-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-216-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-216-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-216-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-216-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-216-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  A7-216-T025 Alexa Fluor® 700 25 tests yes choose region PDF datasheetHTML datasheet buy
  A7-216-T100 Alexa Fluor® 700 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Specificity

The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Regulatory Status

Immunogen

Leukocytes of a patient suffering from a LGL-type leukemia.

Species Reactivity:

  • Human

Negative Species:

  • Canine (Dog)

Applications:

  • Flow Cytometry
    Recommended dilution: 2 μg/ml
  • Immunoprecipitation
  • Western Blotting
    Recommended dilution: 2 μg/ml
    Positive control: Kg-1a human leukemia cell lysate
    Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS
    Application note: Non-reducing conditions. SDS-PAGE (6% separating gel).
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Positive tissue: spleen, microglia
  • Immunohistochemistry (frozen sections)
  • Functional Application
    The antibody MEM-48 induces high-affinity conformation of LFA-1 complex.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Gao JX, Issekutz AC: Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. Immunology. 1996 Jul;88(3):463-70. [Abstract] [Full Text]
  • *Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V: Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun. 2000 Aug 28;275(2):295-9. [Abstract]
  • *Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol. 1998 Jun;28(6):1970-9. [Abstract]
  • *Kess D, Peters T, Zamek J, Wickenhauser C, Tawadros S, Loser K, Varga G, Grabbe S, Nischt R, Sunderkötter C, Müller W, Krieg T, Scharffetter-Kochanek K: CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J Immunol. 2003 Dec 1;171(11):5697-706. [Abstract] [Full Text]
  • *Solovjov DA, Pluskota E, Plow EF: Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2. J Biol Chem. 2005 Jan 14;280(2):1336-45. [Abstract] [Full Text]
  • *Peters T, Sindrilaru A, Wang H, Oreshkova T, Renkl AC, Kess D, Scharffetter-Kochanek K: CD18 in monogenic and polygenic inflammatory processes of the skin. J Investig Dermatol Symp Proc. 2006 Sep;11(1):7-15. [Abstract]
  • *Dassanayake RP, Maheswaran SK, Srikumaran S: Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects. Infect Immun. 2007 Oct;75(10):5004-10. [Abstract]
  • Product Specific References

  • *Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha). 1990;36(1):41-50. [Abstract]
  • *Larson RS, Springer TA: Structure and function of leukocyte integrins. Immunol Rev. 1990 Apr;114:181-217.
  • *Stefanova I, Horejsi V: Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules. J Immunol. 1991 Sep 1;147(5):1587-92. [Abstract]
  • *Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95). J Immunol. 2000 Jun 1;164(11):5928-34. [Abstract] [Full Text]
  • *Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15;93(10):3505-11. [Abstract] [Full Text]
  • *Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT: Increased phagocyte Fc gammaRI expression and improved Fc gamma-receptor-mediated phagocytosis after in vivo recombinant human interferon-gamma treatment of normal human subjects. Blood. 1997 Oct 15;90(8):3187-94. [Abstract] [Full Text]
  • *Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113(2):358-69. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MEM-48 A
    Fig. 1A Flow Cytometry analysis


    Fig. 1 Surface staining of human peripheral blood cells with anti-human CD18 (MEM-48) PE. Cells in the granulocyte gate were used for analysis.


    MEM-48 B
    Fig. 1B Western Blotting


    Fig. 2 Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 (MEM-48).
    Lane 1: KG-1a human acute myeloid leukemia cell line
    Lane 2: HPB-ALL human peripheral blood T cell leukemia cell line

    MEM-48 IHC
    Fig. 3.


    Fig. 3 Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).
    Commercially tested by LifeSpan BioSciences.


    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy