categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD16 / FcgammaRIII

MEM-154 (IgG1)

Product No.	Form	Quantity	Price
11-214-C025	purified	0.025 mg	choose region
11-214-C100	purified	0.1 mg	choose region
10-214-C025	azide free	0.025 mg	choose region
10-214-C100	azide free	0.1 mg	choose region
1B-214-C025	biotin	0.025 mg	choose region
1B-214-C100	biotin	0.1 mg	choose region
1F-214-T025	FITC	25 tests	choose region
1F-214-T100	FITC	100 tests	choose region
1P-214-T025	PE	25 tests	choose region
1P-214-T100	PE	100 tests	choose region
A7-214-T025	Alexa Fluor® 700	25 tests	choose region
A7-214-T100	Alexa Fluor® 700	100 tests	choose region

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Background

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Entrez Gene: 1q23

Specificity

The antibody MEM-154 reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes.
HLDA V; WS Code M MA068
HLDA V; WS Code NK NK51

Immunogen

Human granulocytes

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution: 5-10 μg/ml Positive control: PBL (peripheral blood lymphocytes) Application note: The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Immunoprecipitation Western Blotting Application note: Non-reducing conditions. Functional Application The antibody MEM-154 blocks binding of human IgG to FcgammaRIII. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J Biol Chem. 1995 Jan 20;270(3):1350-61. [Abstract] [Full Text] Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15;159(8):3940-8. [Abstract] Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE-mediated activation of NK cells through Fc gamma RIII. J Immunol. 2003 Mar 15;170(6):3054-8. [Abstract] [Full Text] Product Specific References Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). de Haas M, Koene HR, Kleijer M, de Vries E, Simsek S, van Tol MJ, Roos D, von dem Borne AE: A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa. J Immunol. 1996 Apr 15;156(8):3948-55. [Abstract] Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81. [Abstract] Koene HR, Kleijer M, Algra J, Roos D, von dem Borne AE, de Haas M: Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype. Blood. 1997 Aug 1;90(3):1109-14. [Abstract] [Full Text] Gasdaska JR, Sherwood S, Regan JT, Dickey LF: An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab. Mol Immunol. 2012 Mar;50(3):134-41 [Abstract]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 5-10 μg/ml
Positive control: PBL (peripheral blood lymphocytes)
Application note: The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.
Immunoprecipitation
Western Blotting
Application note: Non-reducing conditions.
Functional Application
The antibody MEM-154 blocks binding of human IgG to FcgammaRIII.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J Biol Chem. 1995 Jan 20;270(3):1350-61. [Abstract] [Full Text]

*Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15;159(8):3940-8. [Abstract]

*Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE-mediated activation of NK cells through Fc gamma RIII. J Immunol. 2003 Mar 15;170(6):3054-8. [Abstract] [Full Text]

Product Specific References

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

*de Haas M, Koene HR, Kleijer M, de Vries E, Simsek S, van Tol MJ, Roos D, von dem Borne AE: A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa. J Immunol. 1996 Apr 15;156(8):3948-55. [Abstract]

*Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81. [Abstract]

*Koene HR, Kleijer M, Algra J, Roos D, von dem Borne AE, de Haas M: Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype. Blood. 1997 Aug 1;90(3):1109-14. [Abstract] [Full Text]

*Gasdaska JR, Sherwood S, Regan JT, Dickey LF: An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab. Mol Immunol. 2012 Mar;50(3):134-41 [Abstract]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG1 Isotype Control

Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Surface staining of human peripheral blood cells with anti-human CD16 (MEM-154) PE. The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.

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