System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD16 / FcgammaRIII

MEM-154 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-214-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-214-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  10-214-C025 azide free 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  10-214-C100 azide free 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-214-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-214-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-214-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-214-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-214-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-214-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  A7-214-T025 Alexa Fluor® 700 25 tests yes choose region PDF datasheetHTML datasheet buy
  A7-214-T100 Alexa Fluor® 700 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Specificity

The antibody MEM-154 reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes.
HLDA V; WS Code M MA068
HLDA V; WS Code NK NK51

Immunogen

Human granulocytes

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 5-10 μg/ml
    Positive control: PBL (peripheral blood lymphocytes)
    Application note: The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.
  • Immunoprecipitation
  • Western Blotting
    Application note: Non-reducing conditions.
  • Functional Application
    The antibody MEM-154 blocks binding of human IgG to FcgammaRIII.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Gessner JE, Grussenmeyer T, Kolanus W, Schmidt RE: The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J Biol Chem. 1995 Jan 20;270(3):1350-61. [Abstract] [Full Text]
  • *Kocher M, Siegel ME, Edberg JC, Kimberly RP: Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 1997 Oct 15;159(8):3940-8. [Abstract]
  • *Arase N, Arase H, Hirano S, Yokosuka T, Sakurai D, Saito T: IgE-mediated activation of NK cells through Fc gamma RIII. J Immunol. 2003 Mar 15;170(6):3054-8. [Abstract] [Full Text]
  • Product Specific References

  • *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  • *de Haas M, Koene HR, Kleijer M, de Vries E, Simsek S, van Tol MJ, Roos D, von dem Borne AE: A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa. J Immunol. 1996 Apr 15;156(8):3948-55. [Abstract]
  • *Tamm A, Schmidt RE: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81. [Abstract]
  • *Koene HR, Kleijer M, Algra J, Roos D, von dem Borne AE, de Haas M: Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype. Blood. 1997 Aug 1;90(3):1109-14. [Abstract] [Full Text]
  • *Gasdaska JR, Sherwood S, Regan JT, Dickey LF: An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab. Mol Immunol. 2012 Mar;50(3):134-41 [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MEM-154
    Fig. 1. Flow Cytometry analysis


    Fig. 1. Surface staining of human peripheral blood cells with anti-human CD16 (MEM-154) PE. The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.

    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight™ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy5, Cy5.5 and Cy7 conjugates are made and sold under license from GE Healthcare Bio-Sciences Corp. The conjugates are licensed for non-commercial research use only. Not for therapeutic or in vivo applications. Other use needs license from GE Healthcare Bio-Sciences Corp. under U.S. patent Nos. 5 569 587 and 5 627 027 and other patents pending.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy