System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD15 / Lewis x Blood Group antigen

MEM-158 (IgM)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-213-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-213-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-213-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-213-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PB-213-T025 Pacific Blue™ 25 tests yes choose region PDF datasheetHTML datasheet buy
  PB-213-T100 Pacific Blue™ 100 tests yes choose region PDF datasheetHTML datasheet buy
  1F-213-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-213-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  PO-213-T025 Pacific Orange™ 25 tests yes choose region PDF datasheetHTML datasheet buy
  PO-213-T100 Pacific Orange™ 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-213-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-213-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-213-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-213-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  PC-213-T025 PerCP 25 tests yes choose region PDF datasheetHTML datasheet buy
  PC-213-T100 PerCP 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.

Specificity

The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
HLDA VI; WS Code AS A053

Regulatory Status

Immunogen

Human granulocytes

Species Reactivity:

  • Human

Negative Species:

  • Porcine
  • Bovine
  • Sheep

Applications:

  • Flow Cytometry
  • Immunoprecipitation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep;39(1-2):185-94. [Abstract]
  • *Hakomori S: Le(X) and related structures as adhesion molecules. Histochem J. 1992 Nov;24(11):771-6. [Abstract]
  • *Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1;406(2):333-41. [Abstract]
  • Product Specific References

  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgM Isotype Control
  • Example Data


    MEM-158
    Fig. 1. Flow Cytometry analysis


    Fig. 1. Surface staining (flow cytometry) of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.


    MEM-158 173Yb


    Fig. 2. Surface staining (mass cytometry) of human peripheral blood cells (after ammonium chloride red blood cell lysis) with anti-human CD15 (MEM-158) 173Yb. Gated on singlets.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registred trademarks of Molecular Probes Inc., Eugene, OR, USA. (licence label)
    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy