categories: CD and Related Antigens (Human), Blood Group Antigens (Human)

Mouse Monoclonal to CD15 / Lewis x Blood Group antigen

MEM-158 (IgM)

Product No.	Form	Quantity	Price
11-213-C025	purified	0.025 mg	choose region
11-213-C100	purified	0.1 mg	choose region
1B-213-C025	biotin	0.025 mg	choose region
1B-213-C100	biotin	0.1 mg	choose region
1F-213-T025	FITC	25 tests	choose region
1F-213-T100	FITC	100 tests	choose region
1P-213-T025	PE	25 tests	choose region
1P-213-T100	PE	100 tests	choose region
1A-213-T025	APC	25 tests	choose region
1A-213-T100	APC	100 tests	choose region
A6-213-T025	Alexa Fluor® 647	25 tests	choose region
A6-213-T100	Alexa Fluor® 647	100 tests	choose region

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Background

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.

Entrez Gene: 11q21

Specificity

The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
HLDA VI; WS Code AS A053

Immunogen

Human granulocytes

Species Reactivity: Human Negative Species: Porcine	Applications: Flow Cytometry Immunoprecipitation Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep;39(1-2):185-94. [Abstract] Hakomori S: Le(X) and related structures as adhesion molecules. Histochem J. 1992 Nov;24(11):771-6. [Abstract] Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1;406(2):333-41. [Abstract] Product Specific References Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

Species Reactivity:

Human

Negative Species:

Porcine

Applications:

Flow Cytometry
Immunoprecipitation

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep;39(1-2):185-94. [Abstract]

*Hakomori S: Le(X) and related structures as adhesion molecules. Histochem J. 1992 Nov;24(11):771-6. [Abstract]

*Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1;406(2):333-41. [Abstract]

Product Specific References

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

For research use only. Not for drug, diagnostic or other use.

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Mouse IgM Isotype Control

Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Surface staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.

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