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Mouse Monoclonal to CD14 / LPS-receptor

B-A8 (IgG1)

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  11-304-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-304-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.

Specificity

The antibody B-A8 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.

Regulatory Status

Immunogen

Human monocytes

Species Reactivity:

  • Human

Negative Species:

Applications:

  • ELISA
    The antibody B-A8 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 (cat. no. 1B-212-C100).
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 2-10 μg/ml
    Positive tissue: PML brain sections
    Application note:
    Positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels.
    Heat retrieval of antigen is recommeded.
  • Flow Cytometry
    Recommended dilution:1 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Juan TS, Hailman E, Kelley MJ, Wright SD, Lichenstein HS: Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding. J Biol Chem. 1995 Jul 21;270(29):17237-42. [Abstract] [Full Text]
  • *Lodrup Carlsen KC, Granum B: Soluble CD14: role in atopic disease and recurrent infections, including otitis media. Curr Allergy Asthma Rep. 2007 Nov;7(6):436-43. [Abstract]
  • *Asai Y, Makimura Y, Kawabata A, Ogawa T: Soluble CD14 Discriminates Slight Structural Differences between Lipid As That Lead to Distinct Host Cell Activation. J Immunol. 2007 Dec 1;179(11):7674-83. [Abstract]
  • *Fernßndez-Real JM, Broch M, Richart C, Vendrell J, Lˇpez-Bermejo A, Ricart W: CD14 monocyte receptor, involved in the inflammatory cascade, and insulin sensitivity. J Clin Endocrinol Metab. 2003 Apr;88(4):1780-4. [Abstract] [Full Text]
  • Product Specific References

  • *Goyert SM, Ferrero E, Rettig WJ, Yenamandra AK, Obata F, Le Beau MM: The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors. Science. 1988 Jan 29;239(4839):497-500. [Abstract]
  • *Kuusniemi AM, Lapatto R, Holmberg C, Karikoski R, Rapola J, Jalanko H.: Kidneys with heavy proteinuria show fibrosis, inflammation, and oxidative stress, but no tubular phenotypic change. Kidney Int. 2005 Jul;68(1):121-32. [Abstract]
  • *Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99. [Abstract] [Full Text]
  • *Wiendl H, Mitsdoerffer M, Schneider D, Melms A, Lochmuller H, Hohlfeld R, Weller M: Muscle fibres and cultured muscle cells express the B7.1/2-related inducible co-stimulatory molecule, ICOSL: implications for the pathogenesis of inflammatory myopathies. Brain. 2003 May;126(Pt 5):1026-35. [Abstract] [Full Text]
  • *MatasiŠ R, Dietz AB, Vuk-PavloviŠ S: Dexamethasone inhibits dendritic cell maturation by redirecting differentiation of a subset of cells. J Leukoc Biol. 1999 Dec;66(6):909-14. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

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