categories: CD and Related Antigens (Human), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to CD14 / LPS-receptor

MEM-15 (IgG1)

Product No.	Form	Quantity	Price
11-293-C025	purified	0.025 mg	choose region
11-293-C100	purified	0.1 mg	choose region
1B-293-C025	biotin	0.025 mg	choose region
1B-293-C100	biotin	0.1 mg	choose region
PB-293-T025	Pacific Blue™	25 tests	choose region
PB-293-T100	Pacific Blue™	100 tests	choose region
1F-293-T025	FITC	25 tests	choose region
1F-293-T100	FITC	100 tests	choose region
PO-293-T025	Pacific Orange™	25 tests	choose region
PO-293-T100	Pacific Orange™	100 tests	choose region
1P-293-T025	PE	25 tests	choose region
1P-293-T100	PE	100 tests	choose region
T5-293-T025	PE-DyLight™ 594	25 tests	choose region
T5-293-T100	PE-DyLight™ 594	100 tests	choose region
1A-293-T025	APC	25 tests	choose region
1A-293-T100	APC	100 tests	choose region
PC-293-T025	PerCP	25 tests	choose region
PC-293-T100	PerCP	100 tests	choose region
T9-293-T025	PerCP-Cy™5.5	25 tests	choose region
T9-293-T100	PerCP-Cy™5.5	100 tests	choose region

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Background

CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.

Entrez Gene (human): 5q31.1

Specificity

The antibody MEM-15 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.
The antibody MEM-15 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
HLDA III; WS Code M 252
HLDA IV; WS Code M 113
HLDA IV; WS Code NL 90
HLDA IV; WS Code T 53
HLDA V; WS Code M MA086
HLDA VI; WS Code M MA94

Immunogen

A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

Species Reactivity: Human Non-Human Primates Negative Species:	Applications: Flow Cytometry Recommended dilution:4 μg/ml Immunoprecipitation excellent for immunoprecipitation of CD14 Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Juan TS, Hailman E, Kelley MJ, Wright SD, Lichenstein HS: Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding. J Biol Chem. 1995 Jul 21;270(29):17237-42. [Abstract] [Full Text] Fernández-Real JM, Broch M, Richart C, Vendrell J, López-Bermejo A, Ricart W: CD14 monocyte receptor, involved in the inflammatory cascade, and insulin sensitivity. J Clin Endocrinol Metab. 2003 Apr;88(4):1780-4. [Abstract] [Full Text] Lodrup Carlsen KC, Granum B: Soluble CD14: role in atopic disease and recurrent infections, including otitis media. Curr Allergy Asthma Rep. 2007 Nov;7(6):436-43. [Abstract] Asai Y, Makimura Y, Kawabata A, Ogawa T: Soluble CD14 Discriminates Slight Structural Differences between Lipid As That Lead to Distinct Host Cell Activation. J Immunol. 2007 Dec 1;179(11):7674-83. [Abstract] Product Specific References Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I.: Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen. Eur J Immunol. 1986 Dec;16(12):1583-9. [Abstract] Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987). Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Funda DP, Tucková L, Farré MA, Iwase T, Moro I, Tlaskalová-Hogenová H: CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited. Infect Immun. 2001 Jun;69(6):3772-81. [Abstract] [Full Text] Sing A, Rost D, Tvardovskaia N, Roggenkamp A, Wiedemann A, Kirschning CJ, Aepfelbacher M, Heesemann J: Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression. J Exp Med. 2002 Oct 21;196(8):1017-24. [Abstract] [Full Text] Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT: Increased phagocyte Fc gammaRI expression and improved Fc gamma-receptor-mediated phagocytosis after in vivo recombinant human interferon-gamma treatment of normal human subjects. Blood. 1997 Oct 15;90(8):3187-94. [Abstract] [Full Text]

Species Reactivity:

Human
Non-Human Primates

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:4 μg/ml
Immunoprecipitation
excellent for immunoprecipitation of CD14

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Juan TS, Hailman E, Kelley MJ, Wright SD, Lichenstein HS: Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding. J Biol Chem. 1995 Jul 21;270(29):17237-42. [Abstract] [Full Text]

*Fernández-Real JM, Broch M, Richart C, Vendrell J, López-Bermejo A, Ricart W: CD14 monocyte receptor, involved in the inflammatory cascade, and insulin sensitivity. J Clin Endocrinol Metab. 2003 Apr;88(4):1780-4. [Abstract] [Full Text]

*Lodrup Carlsen KC, Granum B: Soluble CD14: role in atopic disease and recurrent infections, including otitis media. Curr Allergy Asthma Rep. 2007 Nov;7(6):436-43. [Abstract]

*Asai Y, Makimura Y, Kawabata A, Ogawa T: Soluble CD14 Discriminates Slight Structural Differences between Lipid As That Lead to Distinct Host Cell Activation. J Immunol. 2007 Dec 1;179(11):7674-83. [Abstract]

Product Specific References

*Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I.: Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen. Eur J Immunol. 1986 Dec;16(12):1583-9. [Abstract]

*Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).

*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*Funda DP, Tucková L, Farré MA, Iwase T, Moro I, Tlaskalová-Hogenová H: CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited. Infect Immun. 2001 Jun;69(6):3772-81. [Abstract] [Full Text]

*Sing A, Rost D, Tvardovskaia N, Roggenkamp A, Wiedemann A, Kirschning CJ, Aepfelbacher M, Heesemann J: Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression. J Exp Med. 2002 Oct 21;196(8):1017-24. [Abstract] [Full Text]

*Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT: Increased phagocyte Fc gammaRI expression and improved Fc gamma-receptor-mediated phagocytosis after in vivo recombinant human interferon-gamma treatment of normal human subjects. Blood. 1997 Oct 15;90(8):3187-94. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG1 Isotype Control

Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Surface staining of human peripheral blood cells with anti-human CD14 (MEM-15) PE.

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