categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD11b / Mac-1 alpha

MEM-174 (IgG2a)

Product No.	Form	Quantity	Price
11-211-C025	purified	0.025 mg	choose region
11-211-C100	purified	0.1 mg	choose region
10-211-C025	azide free	0.025 mg	choose region
10-211-C100	azide free	0.1 mg	choose region
1B-211-C025	biotin	0.025 mg	choose region
1B-211-C100	biotin	0.1 mg	choose region
1F-211-T025	FITC	25 tests	choose region
1F-211-T100	FITC	100 tests	choose region
1P-211-T025	PE	25 tests	choose region
1P-211-T100	PE	100 tests	choose region
1A-211-T025	APC	25 tests	choose region
1A-211-T100	APC	100 tests	choose region

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Background

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

Entrez Gene: 16p11.2

Specificity

The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
HLDA VI; WS Code BP 310
HLDA VI; WS Code M 18

Immunogen

Human granulocytes

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution:1 μg/ml Positive control: Peripheral Blood Lymphocytes Immunoprecipitation Positive control: Granulocytes CD11b/CD18 transfectants Application note: Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads) It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen). Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95(3):911-20. [Abstract] [Full Text] Lawrence PK, Srikumaran S.: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):287-98. [Abstract] Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E.: Effects of beta-glucans on the immune system. Medicina (Kaunas). 2007;43(8):597-606. [Abstract] Product Specific References Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:1 μg/ml
Positive control: Peripheral Blood Lymphocytes
Immunoprecipitation
Positive control: Granulocytes
CD11b/CD18 transfectants
Application note: Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads)
It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen).

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95(3):911-20. [Abstract] [Full Text]

*Lawrence PK, Srikumaran S.: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):287-98. [Abstract]

*Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E.: Effects of beta-glucans on the immune system. Medicina (Kaunas). 2007;43(8):597-606. [Abstract]

Product Specific References

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG2a Isotype Control

Example Data

Fig. 1. Immunoprecipitation

Fig. 1. Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor.
Lysate was subjected to affinity column chromatography using anti-human CD11b (MEM-174) immunosorbent. Eluted immunoprecipitate was resolved on 7.5% SDS-PAGE and stained with Coomasie Blue.

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