categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD11a / LFA-1 alpha chain

MEM-25 (IgG1)

Product No.	Form	Quantity	Price
11-210-C025	purified	0.025 mg	choose region
11-210-C100	purified	0.1 mg	choose region
10-210-C025	azide free	0.025 mg	choose region
10-210-C100	azide free	0.1 mg	choose region
1B-210-C025	biotin	0.025 mg	choose region
1B-210-C100	biotin	0.1 mg	choose region
PB-210-T025	Pacific Blue™	25 tests	choose region
PB-210-T100	Pacific Blue™	100 tests	choose region
1F-210-T025	FITC	25 tests	choose region
1F-210-T100	FITC	100 tests	choose region
1P-210-T025	PE	25 tests	choose region
1P-210-T100	PE	100 tests	choose region
1T-210-T025	PE-Dyomics 590	25 tests	choose region
1T-210-T100	PE-Dyomics 590	100 tests	choose region
1A-210-T025	APC	25 tests	choose region
1A-210-T100	APC	100 tests	choose region
A6-210-T025	Alexa Fluor® 647	25 tests	choose region
A6-210-T100	Alexa Fluor® 647	100 tests	choose region
A7-210-T025	Alexa Fluor® 700	25 tests	choose region
A7-210-T100	Alexa Fluor® 700	100 tests	choose region

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Background

CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.

Entrez Gene: 16p11.2

Specificity

The antibody MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
HLDA IV; WS Code NL 209

Immunogen

Leukocytes from a pacient suffering from a LGL-type leukaemia.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Immunoprecipitation excellent antibody for immunoaffinity purification of LFA-1 complex Functional Application The antibody MEM-25 partially blocks binding of LFA-1 complex to ICAM-1. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Sarantos MR, Raychaudhuri S, Lum AF, Staunton DE, Simon SI: Leukocyte function-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1. J Biol Chem. 2005 Aug 5;280(31):28290-8. [Abstract] [Full Text] Giblin PA, Lemieux RM: LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics. Curr Pharm Des. 2006;12(22):2771-95. [Abstract] Kellersch B, Kolanus W: Membrane-proximal signaling events in beta-2 integrin activation. Results Probl Cell Differ. 2006;43:245-57. [Abstract] Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity. Life Sci. 2007 May 30;80(24-25):2320-4. [Abstract] Product Specific References Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha). 1990;36(1):41-50. [Abstract] Ottonello L, Epstein AL, Mancini M, Dapino P, Dallegri F: Monoclonal LYM-1 antibody-dependent cytolysis by human neutrophils exposed to GM-CSF: auto-regulation of target cell attack by cathepsin G. J Leukoc Biol. 2004 Jan;75(1):99-105. [Abstract] [Full Text] Simon SI, Cherapanov V, Nadra I, Waddell TK, Seo SM, Wang Q, Doerschuk CM, Downey GP: Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18. J Immunol. 1999 Sep 1;163(5):2891-901. [Abstract] [Full Text] Hajishengallis G, Martin M, Sojar HT, Sharma A, Schifferle RE, DeNardin E, Russell MW, Genco RJ: Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction. Clin Diagn Lab Immunol. 2002 Mar;9(2):403-11. [Abstract] [Full Text] Wang JH, Kwas C, Wu L: Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission. J Virol. 2009 May;83(9):4195-204. [Abstract] [Full Text] Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95). J Immunol. 2000 Jun 1;164(11):5928-34. [Abstract] [Full Text] Mathison RD, Befus AD, Davison JS, Woodman RC: Modulation of neutrophil function by the tripeptide feG. BMC Immunol. 2003 Mar 4;4:3. Epub 2003 Mar 4. [Abstract] [Full Text] Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15;93(10):3505-11. [Abstract] [Full Text] Aubert M, Yoon M, Sloan DD, Spear PG, Jerome KR: The virological synapse facilitates herpes simplex virus entry into T cells. J Virol. 2009 Jun;83(12):6171-83. [Abstract] [Full Text] *And many other.

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Immunoprecipitation
excellent antibody for immunoaffinity purification of LFA-1 complex
Functional Application
The antibody MEM-25 partially blocks binding of LFA-1 complex to ICAM-1.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Sarantos MR, Raychaudhuri S, Lum AF, Staunton DE, Simon SI: Leukocyte function-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1. J Biol Chem. 2005 Aug 5;280(31):28290-8. [Abstract] [Full Text]

*Giblin PA, Lemieux RM: LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics. Curr Pharm Des. 2006;12(22):2771-95. [Abstract]

*Kellersch B, Kolanus W: Membrane-proximal signaling events in beta-2 integrin activation. Results Probl Cell Differ. 2006;43:245-57. [Abstract]

*Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity. Life Sci. 2007 May 30;80(24-25):2320-4. [Abstract]

Product Specific References

*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

*Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha). 1990;36(1):41-50. [Abstract]

*Ottonello L, Epstein AL, Mancini M, Dapino P, Dallegri F: Monoclonal LYM-1 antibody-dependent cytolysis by human neutrophils exposed to GM-CSF: auto-regulation of target cell attack by cathepsin G. J Leukoc Biol. 2004 Jan;75(1):99-105. [Abstract] [Full Text]

*Simon SI, Cherapanov V, Nadra I, Waddell TK, Seo SM, Wang Q, Doerschuk CM, Downey GP: Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18. J Immunol. 1999 Sep 1;163(5):2891-901. [Abstract] [Full Text]

*Hajishengallis G, Martin M, Sojar HT, Sharma A, Schifferle RE, DeNardin E, Russell MW, Genco RJ: Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction. Clin Diagn Lab Immunol. 2002 Mar;9(2):403-11. [Abstract] [Full Text]

*Wang JH, Kwas C, Wu L: Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission. J Virol. 2009 May;83(9):4195-204. [Abstract] [Full Text]

*Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95). J Immunol. 2000 Jun 1;164(11):5928-34. [Abstract] [Full Text]

*Mathison RD, Befus AD, Davison JS, Woodman RC: Modulation of neutrophil function by the tripeptide feG. BMC Immunol. 2003 Mar 4;4:3. Epub 2003 Mar 4. [Abstract] [Full Text]

*Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15;93(10):3505-11. [Abstract] [Full Text]

*Aubert M, Yoon M, Sloan DD, Spear PG, Jerome KR: The virological synapse facilitates herpes simplex virus entry into T cells. J Virol. 2009 Jun;83(12):6171-83. [Abstract] [Full Text]

*And many other.

For research use only. Not for drug, diagnostic or other use.

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Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Surface staining of human peripheral blood cells with anti-human CD11a (MEM-25) PE.

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