categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD10 / CALLA

MEM-78 (IgG1)

Product No.	Form	Quantity	Price
11-209-C025	purified	0.025 mg	choose region
11-209-C100	purified	0.1 mg	choose region
1B-209-C025	biotin	0.025 mg	choose region
1B-209-C100	biotin	0.1 mg	choose region
PB-209-T025	Pacific Blue™	25 tests	choose region
PB-209-T100	Pacific Blue™	100 tests	choose region
1F-209-T025	FITC	25 tests	choose region
1F-209-T100	FITC	100 tests	choose region
1P-209-T025	PE	25 tests	choose region
1P-209-T100	PE	100 tests	choose region
1A-209-T025	APC	25 tests	choose region
1A-209-T100	APC	100 tests	choose region
PC-209-T025	PerCP	25 tests	choose region
PC-209-T100	PerCP	100 tests	choose region
T9-209-T025	PerCP-Cy™5.5	25 tests	choose region
T9-209-T100	PerCP-Cy™5.5	100 tests	choose region
A7-209-T025	Alexa Fluor® 700	25 tests	choose region
A7-209-T100	Alexa Fluor® 700	100 tests	choose region

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Background

CD10 (neutral endopeptidase – NEP, common acute lymphocytic leukemia antigen – CALLA, membrane metallo-endopeptidase – MME, enkefalinase) is a 100-kDa cell surface zinc metalloprotease cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.

Entrez Gene: 3q25.1-q25.2

Specificity

The antibody MEM-78 reacts with CD10 antigen (CALLA - Common acute lymphatic leukemia antigen), a 100 kDa type II integral membrane protein.
HLDA IV; WS Code B 506
HLDA V; WS Code B CD10.4

Immunogen

NALM-6 human pre-B cell line

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution:1 μg/ml Immunoprecipitation Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Suzuki T, Ino K, Kikkawa F, Uehara C, Kajiyama H, Shibata K, Mizutani S: Neutral endopeptidase/CD10 expression during phorbol ester-induced differentiation of choriocarcinoma cells through the protein kinase C- and extracellular signal-regulated kinase-dependent signalling pathway. Placenta. 2002 Jul;23(6):475-82. [Abstract] Yada K, Kashima K, Daa T, Kitano S, Fujiwara S, Yokoyama S: Expression of CD10 in basal cell carcinoma. Am J Dermatopathol. 2004 Dec;26(6):463-71. [Abstract] Braham H, Trimeche M, Ziadi S, Mestiri S, Mokni M, Amara K, Hachana M, Sriha B, Korbi S: CD10 expression by fusiform stromal cells in nasopharyngeal carcinoma correlates with tumor progression. Virchows Arch. 2006 Aug;449(2):220-4. [Abstract] Dall'Era MA, True LD, Siegel AF, Porter MP, Sherertz TM, Liu AY: Differential expression of CD10 in prostate cancer and its clinical implication. BMC Urol. 2007 Mar 2;7:3. [Abstract] [Full Text] Product Specific References Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I.: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34. [Abstract] Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase. Blood. 1999 Feb 15;93(4):1437-9. [Abstract]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:1 μg/ml
Immunoprecipitation

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Suzuki T, Ino K, Kikkawa F, Uehara C, Kajiyama H, Shibata K, Mizutani S: Neutral endopeptidase/CD10 expression during phorbol ester-induced differentiation of choriocarcinoma cells through the protein kinase C- and extracellular signal-regulated kinase-dependent signalling pathway. Placenta. 2002 Jul;23(6):475-82. [Abstract]

*Yada K, Kashima K, Daa T, Kitano S, Fujiwara S, Yokoyama S: Expression of CD10 in basal cell carcinoma. Am J Dermatopathol. 2004 Dec;26(6):463-71. [Abstract]

*Braham H, Trimeche M, Ziadi S, Mestiri S, Mokni M, Amara K, Hachana M, Sriha B, Korbi S: CD10 expression by fusiform stromal cells in nasopharyngeal carcinoma correlates with tumor progression. Virchows Arch. 2006 Aug;449(2):220-4. [Abstract]

*Dall'Era MA, True LD, Siegel AF, Porter MP, Sherertz TM, Liu AY: Differential expression of CD10 in prostate cancer and its clinical implication. BMC Urol. 2007 Mar 2;7:3. [Abstract] [Full Text]

Product Specific References

*Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I.: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34. [Abstract]

*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

*Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase. Blood. 1999 Feb 15;93(4):1437-9. [Abstract]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG1 Isotype Control

Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD10 (MEM-78) PE. Total viable cells were used for analysis.

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