categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD8

MEM-31 (IgG2a)

Product No.	Form	Quantity	Price
11-207-C025	purified	0.025 mg	choose region
11-207-C100	purified	0.1 mg	choose region
1B-207-C025	biotin	0.025 mg	choose region
1B-207-C100	biotin	0.1 mg	choose region
PB-207-T025	Pacific Blue™	25 tests	choose region
PB-207-T100	Pacific Blue™	100 tests	choose region
1F-207-T025	FITC	25 tests	choose region
1F-207-T100	FITC	100 tests	choose region
PO-207-T025	Pacific Orange™	25 tests	choose region
PO-207-T100	Pacific Orange™	100 tests	choose region
1P-207-T025	PE	25 tests	choose region
1P-207-T100	PE	100 tests	choose region
1T-207-T025	PE-Dyomics 590	25 tests	choose region
1T-207-T100	PE-Dyomics 590	100 tests	choose region
T5-207-T025	PE-DyLight™ 594	25 tests	choose region
T5-207-T100	PE-DyLight™ 594	100 tests	choose region
1A-207-T025	APC	25 tests	choose region
1A-207-T100	APC	100 tests	choose region
1Y-207-T025	PE-Dyomics 647	25 tests	choose region
1Y-207-T100	PE-Dyomics 647	100 tests	choose region
PC-207-T025	PerCP	25 tests	choose region
PC-207-T100	PerCP	100 tests	choose region
T9-207-T025	PerCP-Cy™5.5	25 tests	choose region
T9-207-T100	PerCP-Cy™5.5	100 tests	choose region
A7-207-T025	Alexa Fluor® 700	25 tests	choose region
A7-207-T100	Alexa Fluor® 700	100 tests	choose region

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Background

The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Entrez Gene (CD8A): 2p12
Entrez Gene (CD8B): 2p12

Specificity

The antibody MEM-31 recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).
The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application.

HLDA III; WS Code T 575

Immunogen

Crude thymus membrane fraction.

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution:1 μg/ml Immunoprecipitation Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB: Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding. J Immunol. 2006 Sep 15;177(6):3930-8. [Abstract] [Full Text] Pang DJ, Hayday AC, Bijlmakers MJ.: CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers. J Biol Chem. 2007 May 4;282(18):13884-94. [Abstract] van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8-driven modulation of T cell antigen receptor specificity. J Theor Biol. 2007 Nov 21;249(2):395-408. [Abstract] Product Specific References Horejsi V et al.: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol. (Praha) 32, 12 (1986). Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987). Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34. [Abstract] Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62. [Abstract] [Full Text] Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. [Abstract] [Full Text] Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J Immunol. 2007 Apr 1;178(7):4402-10. [Abstract] [Full Text] Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol. 2010;2010:841451. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:1 μg/ml
Immunoprecipitation

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB: Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding. J Immunol. 2006 Sep 15;177(6):3930-8. [Abstract] [Full Text]

*Pang DJ, Hayday AC, Bijlmakers MJ.: CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers. J Biol Chem. 2007 May 4;282(18):13884-94. [Abstract]

*van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8-driven modulation of T cell antigen receptor specificity. J Theor Biol. 2007 Nov 21;249(2):395-408. [Abstract]

Product Specific References

*Horejsi V et al.: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol. (Praha) 32, 12 (1986).

*Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).

*Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34. [Abstract]

*Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62. [Abstract] [Full Text]

*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. [Abstract] [Full Text]

*Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J Immunol. 2007 Apr 1;178(7):4402-10. [Abstract] [Full Text]

*Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol. 2010;2010:841451. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG2a Isotype Control

Example Data

Fig. 1.

Surface staining of human peripheral blood lymphocytes using anti-human CD8 (clone MEM-31) PE.

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