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Mouse Monoclonal to CD6

MEM-98 (IgG1)

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Background

CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.

Specificity

The MEM-98 antibody reacts with CD6, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.

Regulatory Status

Immunogen

Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone).

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 2 μg/ml
  • Immunoprecipitation
  • Western Blotting
    Application note: Non-reducing conditions.
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Positive tissue: spleen
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Osorio LM, De Santiago A, Aguilar-Santelises M, Mellstedt H, Jondal M.: CD6 ligation modulates the Bcl-2/Bax ratio and protects chronic lymphocytic leukemia B cells from apoptosis induced by anti-IgM. Blood. 1997 Apr 15;89(8):2833-41. [Abstract] [Full Text]
  • *Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG.: Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells. Blood. 2006 Apr 15;107(8):3212-20. Epub 2005 Dec 13. [Abstract] [Full Text]
  • Product Specific References

  • *Bazil V. et al: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol. (Praha) 35, 35 (1989).
  • *Hassan NJ, Simmonds SJ, Clarkson NG, Hanrahan S, Puklavec MJ, Bomb M, Barclay AN, Brown MH: CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76. Mol Cell Biol. 2006 Sep;26(17):6727-38. [Abstract] [Full Text]
  • *Castro MA, Oliveira MI, Nunes RJ, Fabre S, Barbosa R, Peixoto A, Brown MH, Parnes JR, Bismuth G, Moreira A, Rocha B, Carmo AM: Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse. J Immunol. 2007 Apr 1;178(7):4351-61. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MEM-98


    Fig. 1. Surface staining of human peripheral blood cells with anti-human CD6 (MEM-98) FITC.



    MEM-98 PE


    Fig. 2. Surface staining of human peripheral blood cells with anti-human CD6 (MEM-98) PE. 

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