categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD6

MEM-98 (IgG1)

Product No.	Form	Quantity	Price
11-205-C025	purified	0.025 mg	choose region
11-205-C100	purified	0.1 mg	choose region
PB-205-T025	Pacific Blue™	25 tests	choose region
PB-205-T100	Pacific Blue™	100 tests	choose region
1P-205-T025	PE	25 tests	choose region
1P-205-T100	PE	100 tests	choose region

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Background

CD6, also known as T12, is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166/ALCAM (activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.

Entrez Gene: 11q13

Specificity

The MEM-98 antibody reacts with CD6, a 100-130 kDa single chain transmembrane glycoprotein expressed on T and B lymphocytes subsets, thymocytes, and acute lymphocytic leukemia cells.

Immunogen

Human CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone).

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution: 2 μg/ml Immunoprecipitation Western Blotting Application note: Non-reducing conditions. Immunohistochemistry (paraffin sections) Recommended dilution: 10 μg/ml Positive tissue: spleen Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Osorio LM, De Santiago A, Aguilar-Santelises M, Mellstedt H, Jondal M.: CD6 ligation modulates the Bcl-2/Bax ratio and protects chronic lymphocytic leukemia B cells from apoptosis induced by anti-IgM. Blood. 1997 Apr 15;89(8):2833-41. [Abstract] [Full Text] Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG.: Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells. Blood. 2006 Apr 15;107(8):3212-20. Epub 2005 Dec 13. [Abstract] [Full Text] Product Specific References Bazil V. et al: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol. (Praha) 35, 35 (1989). Hassan NJ, Simmonds SJ, Clarkson NG, Hanrahan S, Puklavec MJ, Bomb M, Barclay AN, Brown MH: CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76. Mol Cell Biol. 2006 Sep;26(17):6727-38. [Abstract] [Full Text] *Castro MA, Oliveira MI, Nunes RJ, Fabre S, Barbosa R, Peixoto A, Brown MH, Parnes JR, Bismuth G, Moreira A, Rocha B, Carmo AM: Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse. J Immunol. 2007 Apr 1;178(7):4351-61. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 2 μg/ml
Immunoprecipitation
Western Blotting
Application note: Non-reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml
Positive tissue: spleen

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Osorio LM, De Santiago A, Aguilar-Santelises M, Mellstedt H, Jondal M.: CD6 ligation modulates the Bcl-2/Bax ratio and protects chronic lymphocytic leukemia B cells from apoptosis induced by anti-IgM. Blood. 1997 Apr 15;89(8):2833-41. [Abstract] [Full Text]

*Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG.: Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells. Blood. 2006 Apr 15;107(8):3212-20. Epub 2005 Dec 13. [Abstract] [Full Text]

Product Specific References

*Bazil V. et al: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol. (Praha) 35, 35 (1989).

*Hassan NJ, Simmonds SJ, Clarkson NG, Hanrahan S, Puklavec MJ, Bomb M, Barclay AN, Brown MH: CD6 regulates T-cell responses through activation-dependent recruitment of the positive regulator SLP-76. Mol Cell Biol. 2006 Sep;26(17):6727-38. [Abstract] [Full Text]

*Castro MA, Oliveira MI, Nunes RJ, Fabre S, Barbosa R, Peixoto A, Brown MH, Parnes JR, Bismuth G, Moreira A, Rocha B, Carmo AM: Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse. J Immunol. 2007 Apr 1;178(7):4351-61. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG1 Isotype Control

Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Surface staining of human peripheral blood cells with anti-human CD6 (MEM-98) FITC.

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