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Mouse Monoclonal to CD2 / LFA-2

MEM-65 (IgG1)

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Background

CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

Specificity

The antibody MEM-65 recognizes an unique epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T-lymphocytes and NK cells; also expressed by all thymocytes.
HLDA VI; WS Code T 6T-012

Regulatory Status

Immunogen

Human peripheral T cells.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1-5 μg/ml
  • Immunoprecipitation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58. J Biol Chem. 1997 Jun 20;272(25):15782-8. [Abstract] [Full Text]
  • *Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of Cellular Avidity Regulation in CD2-CD58-Mediated T Cell Adhesion. ACS Chem Biol. 2006 Nov 21;1(10):649-58. [Abstract]
  • *Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13(31):4207-13. [Abstract] [Full Text]
  • Product Specific References

  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *Drbal K, Hilgert I, Cebecauer M, Angelisova P, Horejsi V: New monoclonal antibodies to human leucocyte surface molecule CD2. Folia Biol (Praha). 1997;43(6):245-6. [Abstract]
  • *Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8. [Abstract] [Full Text]
  • *Espagnolle N, Depoil D, Zaru R, Demeur C, Champagne E, Guiraud M, Valitutti S: CD2 and TCR synergize for the activation of phospholipase Cgamma1/calcium pathway at the immunological synapse. Int Immunol. 2007 Mar;19(3):239-48. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

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