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Mouse Monoclonal to AGR2+AGR3

AGR3.4 (IgG1)

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Background

AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development.

Specificity

The antibody AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2) proteins (19-20 kDa); secreted cytoplasmic proteins which can serve as markers of carcinogenesis.

Regulatory Status

Immunogen

Purified human AGR3 protein

Species Reactivity:

  • Human
  • Other species Not tested

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution: 1 μg/ml
    Positive control: T47D breast cancer cell line
    Negative control: H1299 lung carcinoma cell line
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:5 μg/ml
    Positive tissue:human colon
  • Immunocytochemistry
    Recommended dilution: 1 μg/ml
    Positive control: T47D breast cancer cell line
    Negative control: H1299 lung carcinoma cell line
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Persson S, Rosenquist M, Knoblach B, Khosravi-Far R, Sommarin M, Michalak M: Diversity of the protein disulfide isomerase family: identification of breast tumor induced Hag2 and Hag3 as novel members of the protein family. Mol Phylogenet Evol. 2005 Sep;36(3):734-40.
  • *Fletcher GC, Patel S, Tyson K, Adam PJ, Schenker M, Loader JA, Daviet L, Legrain P, Parekh R, Harris AL, Terrett JA: hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan. Br J Cancer. 2003 Feb 24;88(4):579-85.
  • *Zhang JS, Gong A, Cheville JC, Smith DI, Young CY: AGR2, an androgen-inducible secretory protein overexpressed in prostate cancer. Genes Chromosomes Cancer. 2005 Jul;43(3):249-59.
  • *Zweitzig DR, Smirnov DA, Connelly MC, Terstappen LW, O'Hara SM, Moran E: Physiological stress induces the metastasis marker AGR2 in breast cancer cells. Mol Cell Biochem. 2007 Dec;306(1-2):255-60.
  • *Ramachandran V, Arumugam T, Wang H, Logsdon CD: Anterior gradient 2 is expressed and secreted during the development of pancreatic cancer and promotes cancer cell survival. Cancer Res. 2008 Oct 1;68(19):7811-8.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    agr wb
    Fig. 1.


    Fig. 1. Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.  


    AGR3-4 IHC
    Fig. 2.


    Fig. 2. Immunohistochemistry staining of human colon (paraffin sections) using anti-AGR2+AGR3 (clone AGR3.4). Commercially tested by LifeSpan BioSciences.

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