categories: Serum & Plasma Proteins (Human)

Mouse Monoclonal to AGR3

AGR3.1 (IgG1)

	Product No.	Form	Quantity	Stock	Price	Datasheet	Shop
	11-560-C025	purified	0.025 mg		choose region
	11-560-C100	purified	0.1 mg		choose region

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Background

AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.

Entrez Gene: 7p21.1

Specificity

The antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa); a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.

Immunogen

Purified human AGR3 protein

Species Reactivity: Human Other species Not tested Negative Species:	Applications: Western Blotting Recommended dilution: 1 μg/ml Positive control: T47D breast cancer cell line Negative control: H1299 lung carcinoma cell line Immunohistochemistry (paraffin sections) Recommended dilution: 1 μg/ml Positive tissue: breast cancer Immunohistochemistry (frozen sections) Recommended dilution: 1 μg/ml Positive tissue: breast cancer Immunocytochemistry Recommended dilution: 1 μg/ml Positive control: T47D breast cancer cell line Negative control: H1299 lung carcinoma cell line Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Persson S, Rosenquist M, Knoblach B, Khosravi-Far R, Sommarin M, Michalak M: Diversity of the protein disulfide isomerase family: identification of breast tumor induced Hag2 and Hag3 as novel members of the protein family. Mol Phylogenet Evol. 2005 Sep;36(3):734-40. Fletcher GC, Patel S, Tyson K, Adam PJ, Schenker M, Loader JA, Daviet L, Legrain P, Parekh R, Harris AL, Terrett JA: hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan. Br J Cancer. 2003 Feb 24;88(4):579-85.

Species Reactivity:

Human
Other species Not tested

Negative Species:

Applications:

Western Blotting
Recommended dilution: 1 μg/ml
Positive control: T47D breast cancer cell line
Negative control: H1299 lung carcinoma cell line
Immunohistochemistry (paraffin sections)
Recommended dilution: 1 μg/ml
Positive tissue: breast cancer
Immunohistochemistry (frozen sections)
Recommended dilution: 1 μg/ml
Positive tissue: breast cancer
Immunocytochemistry
Recommended dilution: 1 μg/ml
Positive control: T47D breast cancer cell line
Negative control: H1299 lung carcinoma cell line

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Persson S, Rosenquist M, Knoblach B, Khosravi-Far R, Sommarin M, Michalak M: Diversity of the protein disulfide isomerase family: identification of breast tumor induced Hag2 and Hag3 as novel members of the protein family. Mol Phylogenet Evol. 2005 Sep;36(3):734-40.

*Fletcher GC, Patel S, Tyson K, Adam PJ, Schenker M, Loader JA, Daviet L, Legrain P, Parekh R, Harris AL, Terrett JA: hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan. Br J Cancer. 2003 Feb 24;88(4):579-85.

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG1 Isotype Control

Example Data

Fig. 1.

Fig. 1. Immunohistochemistry staining of AGR3 with mouse monoclonal AGR3.1 in paraffin-embedded sections of breast carcinoma.

Fig. 2.

Fig. 2. Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.

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